These two volumes record the scientific and clinical work presented at the VIIth International and 3rd European joint symposium on purine and pyrimidine metabolism in man held at the Bournemouth International Conference Centre, Bournemouth, UK, from 30th June to 5th July 1991. The series of international meetings at three yearly intervals have previously been held initially in 1973 in Israel, then Austria, Spain, the Netherlands, USA and Japan. The European society for the Study of Purine and Pyrimidine Metabolism in Man (ESSPPM) which has its own executive and some finance first met in Switzerland in 1987, then in Germany in 1989. The steady evolution of the science in this series of meetings is intellectually satisfying; the subsequent clinical progress is emotionally and economically reassuring. As befits the position of purines and pyrimidines at the centre of biochemistry, there has been steady scientific development into molecular genetics and now onto developmental controls and biochemical pharmacology. The complexities of the immune system are being unravelled but an understanding of the human brain largely eludes us. Laboratory based scientists now predominate over those who work as clinical specialists in vii rheumatology, immunology, oncology and paediatrics. However, there continue to be major clinical objectives since large sections are concerned with major causes of death like ATP depletion, cancer and now AIDS; the laboratory work is providing clinical solutions.

These two volumes record the scientific and clinical work presented at the VIIth International and 3rd European joint symposium on purine and pyrimidine metabolism in man held at the Bournemouth International Conference Centre, Bournemouth, UK, from 30th June to 5th July 1991. The series of international meetings at three yearly intervals have previously been held initially in 1973 in Israel, then Austria, Spain, the Netherlands, USA and Japan. The European society for the Study of Purine and Pyrimidine Metabolism in Man (ESSPPM) which has its own executive and some finance first met in Switzerland in 1987, then in Germany in 1989. The steady evolution of the science in this series of meetings is intellectually satisfying; the subsequent clinical progress is emotionally and economically reassuring. As befits the position of purines and pyrimidines at the centre of biochemistry, there has been steady scientific development into molecular genetics and now onto developmental controls and biochemical pharmacology. The complexities of the immune system are being unravelled but an understanding of the human brain largely eludes us. Laboratory based scientists now predominate over those who work as clinical specialists in vii rheumatology, immunology, oncology and paediatrics. However, there continue to be major clinical objectives since large sections are concerned with major causes of death like ATP depletion, cancer and now AIDS; the laboratory work is providing clinical solutions.

One person in four in the industrialized countries suffers from hyperuricemia and is therefore at risk of developing gouty arthritis, nephrolithiasis, or any of the other consequences of urate deposition. At present, far too little is known about urate deposition and the mechanisms by which it occurs, as well as about its clinical consequences, which include formation of toph; over the helix of the ear or in bones close to joints that have never exhibited an attack, development of bursitis, chronic tendovaginitis leading to carpal tunnel syndrome, and gouty paraplegia. Information on these matters is needed to estimate the risks of hyperuricemia and to determine when therapeutic intervention is indicated. The contributions and discussions in this book, resulting from an international symposium held in December 1990 in the Medizinische Poliklinik in Munich, provide an up-to-date source of current knowledge about hyperuricemia in man and its clinical consequences.

IV Die Autoren der einzelnen Abschnitte werden in Fussnoten ge nannt. Die von ihnen verfassten Beitrage reichen jeweils, bis eine neue Autorennennung erfolgt. Entsprechendes gilt fur die Autoren angaben im Inhaltsverzeichnis. Im Interesse einer ubersichtlichen Gliederung war dieses etwas unorthodoxe Vorgehen notig. Einige ihrer starksten Impulse erhielt die Erforschung der Lipoide durch die Arbeiten unserer Lehrer SIEGFRIED J. THANN HAUSER und ERNST KLENK. Es ist uns eine Ehre, ihnen auch an dieser Stelle fur Anleitung und Vorbild zu danken. Munchen, Marz 1965 NEPOMUK ZOLLNER und DIETRICH EBERHAGEN Inhaltsverzeichnis I. Chemische Einfuhrung . . . . . . . . 1 1. Allgemeine Nomenklaturfragen (D. EBERHAGEN) 1 2. Fettsauren. . . . . . . . . . 2 3. Eigentliche Fette oder Glyceride 7 4. Glycerylather. . . . 9 5. Glycerinphosphatide. . . . 10 6. Sphingolipoide . . . . . . 16 7. Ganglioside (H. WIEGANDT). 19 8. Cholesterin und Cholesterinester (D. EBERHAGEN) 29 9. Gallensauren (B. FROSCH u. H. WAGENER) 31 10. Literaturverzeichnis . . . . . . . . . . . 33 11. Allgemeine Untersuchungsmethoden . 35 1. Extraktion der Lipoide aus dem Serum (N. ZOLLNER) 35 2. Gruppentrennungen der Lipoide (D. EBERHAGEN) . . 39 A. Praparative Verfahren. . . . . . . . . . . . . 41 a) Fraktionierung durch Acetonfallung der Phosphatide. 41 b) Fraktionierung durch Dialyse der Neutralfette . 42 c) Fraktionierung auf chromatographischem Wege 44 d) Chromatographische Auf trennung . . . 46 B. Analytische Verfahren. . . . . . . . . . . 51 a) Chemische Bestimmungen (D. EBERHAGEN) . . 51 b) Chromatographische Untersuchungen (G. WOLFRAM) 51 DunnBchichtchromatographische Trennung der Gesa- lipoide in die Lipoidhauptgruppen 56 3. Untersuchung der Phosphatide 57 A. Praparative Verfahren (H."

Das Buch behandelt alle Aspekte des Purinstoffwechsels sowie den Purimidinstoffwechsel. Die klinischen Abschnitte werden durch Kapitel zu denbiochemischen Grundlagen er- g{nzt. Neben der Terapie von St-rungen des Purinstoffwech- sels wird auch die Bedeutung der Purinanaloga in der medika- ment-sen Therapie diskutiert, wo sie z.B. zur Behandlung der HIV-Infektion eingesetzt werden. Das Buch ist deshalb sowohl f}r denklinisch t{tigen Arzt als auch f}r den Wis- senschaftler interessant.