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Due to the significant contributions of carbohydrates to the
functional diversity of the cell, the challenging study of the
glycome has expanded beyond the research of carbohydrate experts
and into the wider scope of the life sciences. To aid all
scientists now delving into this vital subject area, Glycomics:
Methods and Protocols collects a compendium of detailed laboratory
protocols reflecting the increasing availability of sample
preparation, chromatographic, electrophoretic, mass spectrometric,
and bioinformatic tools specifically designed for the analysis of
glycosylation. Leading researchers in the field address subjects
such as glycoprotein and proteoglycan analysis, glycosylation
structure determination, as well as various approaches to
investigate the interaction between glycans and a variety of
carbohydrate-recognizing proteins in order to aid exploration into
the functional significance of the oligosaccharides. Written in the
highly successful Methods in Molecular Biology (TM) series format,
the chapters include introductions to their respective topics,
lists of the necessary materials and reagents, step-by-step,
readily reproducible protocols, and notes on troubleshooting and
avoiding known pitfalls. Authoritative and cutting-edge, Glycomics:
Methods and Protocols serves as a valuable guide for experimenters
facing the challenges of glycan analysis in hope of providing
further insights into the biology of cell-cell communication and
interaction.
Due to the significant contributions of carbohydrates to the
functional diversity of the cell, the challenging study of the
glycome has expanded beyond the research of carbohydrate experts
and into the wider scope of the life sciences. To aid all
scientists now delving into this vital subject area, Glycomics:
Methods and Protocols collects a compendium of detailed laboratory
protocols reflecting the increasing availability of sample
preparation, chromatographic, electrophoretic, mass spectrometric,
and bioinformatic tools specifically designed for the analysis of
glycosylation. Leading researchers in the field address subjects
such as glycoprotein and proteoglycan analysis, glycosylation
structure determination, as well as various approaches to
investigate the interaction between glycans and a variety of
carbohydrate-recognizing proteins in order to aid exploration into
the functional significance of the oligosaccharides. Written in the
highly successful Methods in Molecular Biology (TM) series format,
the chapters include introductions to their respective topics,
lists of the necessary materials and reagents, step-by-step,
readily reproducible protocols, and notes on troubleshooting and
avoiding known pitfalls. Authoritative and cutting-edge, Glycomics:
Methods and Protocols serves as a valuable guide for experimenters
facing the challenges of glycan analysis in hope of providing
further insights into the biology of cell-cell communication and
interaction.
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