This thesis explores the ability of M. maripaludis to capture and convert CO2 to methane in the presence of free nitrogen, and offers a consolidated review of the metabolic processes and applications of M. maripaludis. Further, it develops, validates and analyzes the first genome-scale metabolic model (iMM518) of M. maripaludis. Readers will discover, for the first time, the impact of nitrogen fixation on methane production. As such, the thesis will be of interest to researchers working on M. maripaludis, biofuels and bioenergy, systems biology modeling and its experimental validation, estimation of maintenance energy parameters, nitrogen fixing microbes, and bioremediation.

This thesis explores the ability of M. maripaludis to capture and convert CO2 to methane in the presence of free nitrogen, and offers a consolidated review of the metabolic processes and applications of M. maripaludis. Further, it develops, validates and analyzes the first genome-scale metabolic model (iMM518) of M. maripaludis. Readers will discover, for the first time, the impact of nitrogen fixation on methane production. As such, the thesis will be of interest to researchers working on M. maripaludis, biofuels and bioenergy, systems biology modeling and its experimental validation, estimation of maintenance energy parameters, nitrogen fixing microbes, and bioremediation.