This volume contains the proceedings of the Workshop on Motivic Homotopy Theory and Refined Enumerative Geometry, held from May 14-18, 2018, at the Universitat Duisburg-Essen, Essen, Germany. It constitutes an accessible yet swift introduction to a new and active area within algebraic geometry, which connects well with classical intersection theory. Combining both lecture notes aimed at the graduate student level and research articles pointing towards the manifold promising applications of this refined approach, it broadly covers refined enumerative algebraic geometry.