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The huge potential for gene therapy to cure a wide range of
diseases has led to high expectations and a great increase in
research efforts in this area, particularly in the study of
delivery via viral vectors, widely considered to be more efficient
than DNA transfection. In Viral Vectors for Gene Therapy: Methods
and Protocols, experts in the field present a collection of their
knowledge and experience featuring methodologies that involve virus
production, transferring protocols, and evaluating the efficacy of
gene products. While thoroughly covering the most popular viral
vector systems of adenovirus, retrovirus, and adeno-associated
virus, this detailed volume also explores less common viral vector
systems such as baculovirus, herpes virus, and measles virus, the
growing interest in which is creating a considerable demand for
large scale manufacturing and purification procedures. Written in
the highly successful Methods in Molecular Biology (TM) series
format, many chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and vital
tips on troubleshooting and avoiding known pitfalls. Comprehensive
and practical, Viral Vectors for Gene Therapy: Methods and
Protocols provides basic principles accessible to scientists from a
wide variety of backgrounds for the development of gene therapy
viral products that are safe and effective.
Animal cell technology is becoming an increasingly important part
of biotechnology and many products are now used in human health
care and for veterinary applications. However, there are many times
more products actually in the developmental pipelines of the
biotechnology industry, including various phases of clinical
trials. The Proceedings of the 15th Meeting of the European Society
for Animal Cell Technology (Tours, France, September 1997) presents
the actual current state as well as New Developments and
Applications in Animal Cell Technology for the benefit of society.
These Proceedings represent both the current state and applications
of animal cell technology and the way the technology is expanding
into new areas to give a unique insight into new products and
applications for human and animal health care.
The huge potential for gene therapy to cure a wide range of
diseases has led to high expectations and a great increase in
research efforts in this area, particularly in the study of
delivery via viral vectors, widely considered to be more efficient
than DNA transfection. In Viral Vectors for Gene Therapy: Methods
and Protocols, experts in the field present a collection of their
knowledge and experience featuring methodologies that involve virus
production, transferring protocols, and evaluating the efficacy of
gene products. While thoroughly covering the most popular viral
vector systems of adenovirus, retrovirus, and adeno-associated
virus, this detailed volume also explores less common viral vector
systems such as baculovirus, herpes virus, and measles virus, the
growing interest in which is creating a considerable demand for
large scale manufacturing and purification procedures. Written in
the highly successful Methods in Molecular Biology (TM) series
format, many chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and vital
tips on troubleshooting and avoiding known pitfalls. Comprehensive
and practical, Viral Vectors for Gene Therapy: Methods and
Protocols provides basic principles accessible to scientists from a
wide variety of backgrounds for the development of gene therapy
viral products that are safe and effective.
Animal cell technology is becoming an increasingly important part
of biotechnology and many products are now used in human health
care and for veterinary applications. However, there are many times
more products actually in the developmental pipelines of the
biotechnology industry, including various phases of clinical
trials. The Proceedings of the 15th Meeting of the European Society
for Animal Cell Technology (Tours, France, September 1997) presents
the actual current state as well as New Developments and
Applications in Animal Cell Technology for the benefit of society.
These Proceedings represent both the current state and applications
of animal cell technology and the way the technology is expanding
into new areas to give a unique insight into new products and
applications for human and animal health care.
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Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology - Selected articles from the Meeting of the EFB Section on Microbial Physiology, Semmering, Austria, 5th-8th October 2000 (Paperback, Softcover reprint of hardcover 1st ed. 2001)
Otto-Wilhelm Merten, D. Mattanovich, C. Lang, G. Larsson, P. Neubauer, …
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R5,771
Discovery Miles 57 710
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Ships in 10 - 15 working days
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More then 20 years have passed now since the first recombinant
protein producing microorganisms have been developed. In the
meanwhile, numerous proteins have been produced in bacteria, yeasts
and filamentous fungi, as weIl as higher eukaryotic cells, and even
entire plants and animals. Many recombinant proteins are on the
market today, and some of them reached substantial market volumes.
On the first sight one would expect the technology - including the
physiology of the host strains - to be optimised in detail after a
20 year's period of development. However, several constraints have
limited the incentive for optimisation, especially in the
pharmaceutical industry like the urge to proceed quickly or the
requirement to define the production parameters for registration
early in the development phase. The additional expenses for
registration of a new production strain often prohibits a change to
an optimised strain. A continuous optimisation of the entire
production process is not feasible for the same reasons.
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Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology - Selected articles from the Meeting of the EFB Section on Microbial Physiology, Semmering, Austria, 5th-8th October 2000 (Hardcover, 2001 ed.)
Otto-Wilhelm Merten, D. Mattanovich, C. Lang, G. Larsson, P. Neubauer, …
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R6,016
Discovery Miles 60 160
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Ships in 10 - 15 working days
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The general field of fundamental and applied biotechnology becomes
increasingly important for the production of biologicals for human
and veterinary use, by using prokaryotic and eukaryotic
microorganisms. The papers in the present book are refereed
articles compiled from oral and poster presentations from the EFB
Meeting on Recombinant Protein Production with Prokaryotic and
Eukaryotic Cells. A Comparative View on Host Physiology, which was
organized in Semmering/A from 5th to 8th October 2000. A special
feature of this meeting was the comparison of different classes of
host cells, mainly bacteria, yeasts, filamentous fungi, and animal
cells, which made obvious that many physiological features of
recombinant protein formation, like cell nutrition, stress
responses, protein folding and secretion, or genetic stability,
follow similar patterns in different expression systems. This
comparative aspect is by far the point of most interest because
such comparisons are rarely done, and if they are done, their
results are most often kept secret by the companies who generated
them. Audience: Presently, a comparable book does not exist because
the compiling of manuscripts from all fields of biotechnology
(prokaryotic as well as eukaryotic, up to animal cell
biotechnology) is not done in general. This particularity makes
this book very interesting for postgraduate students and
professionals in the large field of biotechnology who want to get a
more global view on the current state of the expression of
recombinant biologicals in different host cell systems, the
physiological problems associated with the use of different
expression systems, potential approaches to solve such difficulties
bymetabolic engineering or the use of other host cells, and the
cooperation between process development and strain improvement,
which is crucial for the optimisation of both the production strain
and the process. This book should be in every library of an
institution/organization involved in biotechnology.
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