This volume focuses on mono-ADP-ribosylation and enzymes that use NAD+ including Sirtuins, PARPs, and bacterial and eukaryotic ADP-ribosyltransferases. The chapters in this book are organized into eight parts, and offer detailed descriptions of key protocols used to study topics such as in vitro techniques for ADP-ribosylation substrate identification; biochemical and biophysical assays of PAR-WWE domain interactions; monitoring expression and enzyme activity of ecto-ARTCs; HPLC-based enzymes assays for Sirtuins; and identifying target RNAs of PARPs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, ADP-ribosylation and NAD+ Utilizing Enzymes: Methods and Protocols is a valuable resource for anyone interested in this developing and expanding field.

This volume includes a series of protocols focused on mitotic spindle assembly and function. The methods covered in this book feature a broad range of techniques from basic microscopy to the study of spindle physiologies relevant to cancer. These methods can be applied to diverse model systems that range from the cell-free Xenopus egg extract system to the moss Physcomitrella patens, in an effort to demonstrate the key contributions made by researchers using multiple model organisms. Chapters in The Mitotic Spindle: Methods and Protocols integrate cutting-edge technologies that have only become available due to the cross-disciplinary efforts, such as ATP analogue sensitive inhibition of mitotic kinases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and informative, The Mitotic Spindle: Methods and Protocols, is a valuable resource for researchers who are new to mitosis or are already experts in the field.

1970s South Korea is characterized by many as the "dark age for democracy." Most scholarship on South Korea's democracy movement and civil society has focused on the "student revolution" in 1960 and the large protest cycles in the 1980s which were followed by Korea's transition to democracy in 1987. But in his groundbreaking work of political and social history of 1970s South Korea, Paul Chang highlights the importance of understanding the emergence and evolution of the democracy movement in this oft-ignored decade. Protest Dialectics journeys back to 1970s South Korea and provides readers with an in-depth understanding of the numerous events in the 1970s that laid the groundwork for the 1980s democracy movement and the formation of civil society today. Chang shows how the narrative of the 1970s as democracy's "dark age" obfuscates the important material and discursive developments that became the foundations for the movement in the 1980s which, in turn, paved the way for the institutionalization of civil society after transition in 1987. To correct for these oversights in the literature and to better understand the origins of South Korea's vibrant social movement sector this book presents a comprehensive analysis of the emergence and evolution of the democracy movement in the 1970s.

1970s South Korea is characterized by many as the "dark age for democracy." Most scholarship on South Korea's democracy movement and civil society has focused on the "student revolution" in 1960 and the large protest cycles in the 1980s which were followed by Korea's transition to democracy in 1987. But in his groundbreaking work of political and social history of 1970s South Korea, Paul Chang highlights the importance of understanding the emergence and evolution of the democracy movement in this oft-ignored decade. Protest Dialectics journeys back to 1970s South Korea and provides readers with an in-depth understanding of the numerous events in the 1970s that laid the groundwork for the 1980s democracy movement and the formation of civil society today. Chang shows how the narrative of the 1970s as democracy's "dark age" obfuscates the important material and discursive developments that became the foundations for the movement in the 1980s which, in turn, paved the way for the institutionalization of civil society after transition in 1987. To correct for these oversights in the literature and to better understand the origins of South Korea's vibrant social movement sector this book presents a comprehensive analysis of the emergence and evolution of the democracy movement in the 1970s.

This volume includes a series of protocols focused on mitotic spindle assembly and function. The methods covered in this book feature a broad range of techniques from basic microscopy to the study of spindle physiologies relevant to cancer. These methods can be applied to diverse model systems that range from the cell-free Xenopus egg extract system to the moss Physcomitrella patens, in an effort to demonstrate the key contributions made by researchers using multiple model organisms. Chapters in The Mitotic Spindle: Methods and Protocols integrate cutting-edge technologies that have only become available due to the cross-disciplinary efforts, such as ATP analogue sensitive inhibition of mitotic kinases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and informative, The Mitotic Spindle: Methods and Protocols, is a valuable resource for researchers who are new to mitosis or are already experts in the field.