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Molecular techniques are emerging as a rapid and powerful approach
for microorganism detection. This project reviewed the efficacy of
PCR assays for Cryptosporidium, Microcystis, adenovirus and ammonia
oxidising bacteria, as well as candidate techniques for DNA
extraction and inhibitor removal. The literature review led to an
evaluation of DNA extraction kits and reagents for PCR;
finalisation of assay formats; the development of PCR controls and
Standard Operating Procedures (SOP); analysis of assay robustness
using real samples; and completion of an Inter-laboratory trial
using the SOP's developed. This book is co-published with Water
Research Australia.
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