Amino Acid Analysis (AAA) is an integral part of analytical biochemistry. In a relatively short time, the variety of AAA methods has evolved dramatically with more methods shifting to the use of mass spectrometry (MS) as a detection method. Another new aspect is miniaturization. However, most importantly, AAA in this day and age should be viewed in the context of Metabolomics as a part of Systems Biology. Amino Acid Analysis: Methods and Protocols presents a broad spectrum of all available methods allowing for readers to choose the method that most suits their particular laboratory set-up and analytical needs. In this volume, a reader can find chapters describing general as well as specific approaches to the sample preparation. A number of chapters describe specific applications of AAA in clinical chemistry as well as in food analysis, microbiology, marine biology, drug metabolism, even archeology. Separate chapters are devoted to the application of AAA for protein quantitation and chiral AAA. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Amino Acid Analysis: Methods and Protocols provides crucial techniques that can be applied across multiple disciplines by anyone involved in biomedical research or life sciences.

Amino Acid Analysis (AAA) is an integral part of analytical biochemistry. In a relatively short time, the variety of AAA methods has evolved dramatically with more methods shifting to the use of mass spectrometry (MS) as a detection method. Another new aspect is miniaturization. However, most importantly, AAA in this day and age should be viewed in the context of Metabolomics as a part of Systems Biology. Amino Acid Analysis: Methods and Protocols presents a broad spectrum of all available methods allowing for readers to choose the method that most suits their particular laboratory set-up and analytical needs. In this volume, a reader can find chapters describing general as well as specific approaches to the sample preparation. A number of chapters describe specific applications of AAA in clinical chemistry as well as in food analysis, microbiology, marine biology, drug metabolism, even archeology. Separate chapters are devoted to the application of AAA for protein quantitation and chiral AAA. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Amino Acid Analysis: Methods and Protocols provides crucial techniques that can be applied across multiple disciplines by anyone involved in biomedical research or life sciences.

Made-for-TV romantic drama starring Candace Cameron Bure and Victor Webster. Single mother Megan (Cameron Bure) adopts a dog for her young daughter Caitlin (Katie Hawkins) who has been struggling to make friends since moving to a new neighbourhood. The dog, Jake, creates chaos in Megan's home but Caitlin quickly forms a strong bond with him. Jake's real owner Ben (Webster) turns up and explains that the dog ran off when the garden gate was left open but when he sees how attached Caitlin is to her canine friend Ben agrees to share him with her. As Megan and Ben spend more time in each other's company they begin to develop romantic feelings for one another. Will both he and Jake become a permanent part of the family?

Stacia Altman has just received some terrible news. Life-altering news. In fact, life-ending news. She was just diagnosed with a terminal disease. Worse yet, she's not sure if her death will matter to anyone-including herself. Once an adventurous young girl with a passion for art history, Stacia has somehow evolved into a sarcastic and listless 38-year-old wife of an ultra-controlling husband-a process she didn't even realize was taking place until it was too late. Instead of lying down on the tracks and waiting for a train to run her over, Stacia decides to abandon the societal role she has played, in order to piece together whatever shreds of her life she can, before it's too late. While fighting her way through the stages of grieving, Stacia finds great friends, love, and most importantly, humor. As she travels the globe solving the mystery of her life and coming to terms with her inevitable death, she ultimately discovers that the end might just be the beginning...of a greater adventure. Follow Stacia on her journey of spiritual awakening, physical pleasure, and discovery of self-a tale told from within her urn.