This volume provides a comprehensive and technical presentation of numerous aspects of reproductive cell tissue cryopreservation, and presents readers with current procedures and detailed discussions of novel techniques and the latest innovations. The chapters in this book are divided into five parts and cover subjects such as: immature oocyte cryopreservation, human sperm vitrification and slow-freezing, directional freezing of ovarian tissue, automated vitrification systems, and detailed protocols on popular and commercially available cryopreservation/vitrification systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Cryopreservation of Mammalian Gametes and Embryos: Methods and Protocols includes chapters written by leading experts in the field and is a valuable resource for anyone interested in the field of cryopreservation.

This new paperback edition of the The World Encyclopedia of Contemporary Theatre: Europe covers theatre since World War II in forty-seven European nations, including the nations which re-emerged following the break-up of the former USSR, Czechoslovakia and Yugoslavia. Each national article is divided into twelve sections - History, Structure of the National Theatre Community, Artistic profile, Music Theatre, Theatre for Young Audiences, Puppet Theatre, Design, Theatre, Space and Architecture, Training, Criticism, Scholarship and Publishing and Further Reading - allowing the reader to use the book as a source for both area and subject studies. A new preface and further reading sections by the Series Editor brings the Encyclopedia bang up-to-date making it invaluable to anyone interested in European theatre, as well as students and scholars of performance studies, history, anthropology and cultural studies.

This new paperback edition of the The World Encyclopedia of Contemporary Theatre: Europe covers theatre since World War II in forty-seven European nations, including the nations which re-emerged following the break-up of the former USSR, Czechoslovakia and Yugoslavia. Each national article is divided into twelve sections - History, Structure of the National Theatre Community, Artistic profile, Music Theatre, Theatre for Young Audiences, Puppet Theatre, Design, Theatre, Space and Architecture, Training, Criticism, Scholarship and Publishing and Further Reading - allowing the reader to use the book as a source for both area and subject studies.
A new preface and further reading sections by the Series Editor brings the Encyclopedia bang up-to-date making it invaluable to anyone interested in European theatre, as well as students and scholars of performance studies, history, anthropology and cultural studies.

This concise, truncated version of Nagy, Varghese and Agarwal's Practical Manual of In Vitro Fertilization is comprised of select clinical chapters for a portable, affordable and up-to-date resource. Gamete Assessment, Selection and Micromanipulation in ART covers a variety of topics, including: - Human oocyte evaluation - Sperm evaluation and selection - Micromanipulators and micromanipulation - Biopsy procedures on oocytes and embryos - Molecular insights in IVF Practical for both clinicians and researchers alike, Gamete Assessment, Selection and Micromanipulation in ART brings together all of the need-to-know information about these important topics in reproductive medicine.

This concise, truncated version of Nagy, Varghese and Agarwal's Practical Manual of In Vitro Fertilization is comprised of select clinical chapters for a portable, affordable and up-to-date resource. Clinical Embryology covers a variety of topics, including: - Sperm processing methods - Embryo culture methods - Insemination procedures - Embryo evaluation - Genetic testing of embryos - Cryopreservation - Embryo transfer Also included are two new chapters on predicting embryo developmental potential and viability using automated time-lapse analysis (Eeva) and predicting embryo implantation potential using Embryoscope. (TM) Practical for both clinicians and researchers alike, Clinical Embryology brings together all of the need-to-know information about these important topics in reproductive medicine.

Following the considerable success of the first edition of Plant Virology Protocols, this exciting new edition covers the many new techniques that are now applied to the examination and understanding of plant viruses. Each section presents the most novel methods and step-by-step reproducible laboratory protocols to allow researchers more effective approaches to study plant viruses. This updated book will prove indispensable to laboratory investigators studying plant viruses.

This volume provides a comprehensive and technical presentation of numerous aspects of reproductive cell tissue cryopreservation, and presents readers with current procedures and detailed discussions of novel techniques and the latest innovations. The chapters in this book are divided into five parts and cover subjects such as: immature oocyte cryopreservation, human sperm vitrification and slow-freezing, directional freezing of ovarian tissue, automated vitrification systems, and detailed protocols on popular and commercially available cryopreservation/vitrification systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Cryopreservation of Mammalian Gametes and Embryos: Methods and Protocols includes chapters written by leading experts in the field and is a valuable resource for anyone interested in the field of cryopreservation.

The Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices is a unique, accessible title that provides a complete review of the most well-established and current diagnostic and treatment techniques comprising in vitro fertilization. Throughout the chapters, a uniform structure is employed, including a brief abstract, a keyword glossary, a step-by-step protocol of the laboratory procedures, several pages of expert commentary, key issues of clinical concern, and a list of references. The result is a readily accessible, high quality reference guide for reproductive endocrinologists, urologists, embryologists, biologists and research scientists. The Manual also offers an excellent description of novel procedures that will likely be employed in the near future. An indispensable resource for physicians and basic scientists, the Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices is an invaluable reference and addition to the literature.

This concise, truncated version of Nagy, Varghese and Agarwal's Practical Manual of In Vitro Fertilization is comprised of select practical chapters for a portable, affordable and up-to-date resource. Building and Managing an IVF Laboratory covers a variety of topics, including: - Setting up and running an IVF laboratory - IVF laboratory equipment and culture systems - Organization of the IVF unit - Licensing and regulation in the ART laboratory - Quality control and troubleshooting Practical for both clinicians and researchers alike, Building and Managing an IVF Laboratory brings together all of the need-to-know information about these important topics in reproductive medicine.

Physical Ultrasonics of Composites is a rigorous introduction to the characterization of composite materials by means of ultrasonic waves. Composites are treated here not simply as uniform media, but as inhomogeneous layered anisotropic media with internal structure characteristic of composite laminates. The objective here is to concentrate on exposing the singular behavior of ultrasonic waves as they interact with layered, anisotropic materials, materials which incorporate those structural elements typical of composite laminates.
This book provides a synergistic description of both modeling and experimental methods in addressing wave propagation phenomena and composite property measurements. After a brief review of basic composite mechanics, a thorough treatment of ultrasonics in anisotropic media is presented, along with composite characterization methods. The interaction of ultrasonic waves at interfaces of anisotropic materials is discussed, as are guided waves in composite plates and rods. Waves in layered media are developed from the standpoint of the "Stiffness Matrix," a major advance over the conventional, potentially unstable Transfer Matrix approach. Laminated plates are treated both with the stiffness matrix and using Floquet analysis. The important influence on the received electronic signals in ultrasonic materials characterization from transducer geometry and placement are carefully exposed in a dedicated chapter. Ultrasonic wave interactions are especially susceptible to such influences because ultrasonic transducers are seldom more than a dozen or so wavelengths in diameter. The book ends with a chapter devoted to the emerging field of air-coupled ultrasonics. This new technology has come of age with the development of purpose-built transducers and electronics and is finding ever wider applications, particularly in the characterization of composite laminates.

For around half of the couples who have trouble conceiving the cause of infertility is sperm-related. Intracytoplasmic sperm injection (ICSI) is the most common and successful treatment for male infertility. Here, the pioneers for the technique, along with authorities in the field, describe the underlying science of ICSI and other micromanipulation techniques. Practical advice for performing the techniques is covered in depth, including sperm selection, laser-assisted ICSI, and the use of piezo in ICSI. Examining the safety of ICSI in animal models as well as the impact of ICSI on the health and well-being of the children conceived through the procedure is discussed. This manual is an essential resource for clinical embryologists and laboratory personnel wishing to refine or develop techniques and improve outcomes.

Now in its revised and expanded second edition - including over 20 new chapters - this comprehensive textbook remains a unique and accessible description of the current and developing diagnostic and treatment techniques and technologies comprising in vitro fertilization (IVF). Arranged thematically in sections, each chapter covers a key topic in IVF in a sensible presentation. Parts one and two describe the planning, design and organization of an ART unit and IVF laboratory and equipment and systems, respectively. The sections that follow provide detailed descriptions of IVF techniques, embryo culture methods, sperm processing and selection, insemination procedures, micromanipulation, embryo evaluation, cryopreservation, and embryo transfer. Concluding sections address issues of management and regulation of ART labs across the globe, as well as special topics and emerging techniques and devices. Chapter authors, all experts in the field, contribute their expertise from around the world. With the addition of learning key points and review questions at the beginning and end of each chapter, this new edition of In Vitro Fertilization is a readily accessible, high quality instructional resource for reproductive medicine trainees at all levels. Practicing reproductive endocrinologists, urologists, and embryologists also will find value in the book, as will infertility researchers.