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This volume provides a wide range of protocols used in studying the
nuclear envelope, with special attention to the experimental
adjustments that may be required to successfully investigate this
complex organelle in cells from various organisms. The Nuclear
Envelope: Methods and Protocols is divided into five sections: Part
I - Nuclear Envelope Isolation; Part II - Nuclear Envelope Protein
Interactions, Localization, and Dynamics; Part III - Nuclear
Envelope Interactions with the Cytoskeleton; Part IV - Nuclear
Envelope-Chromatin Interactions; and Part V - Nucleo-Cytoplasmic
Transport. Many of the modifications discussed in this book have
only been circulated within laboratories that have conducted
research in this field for many years. Written in the highly
successful Methods in Molecular Biology series format, chapters
include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Cutting edge and thorough, The Nuclear
Envelope: Methods and Protocols is a timely resource for
researchers who have joined this dynamic and rapidly growing field.
This volume provides an exhaustive review of the most current
knowledge of the composition, architecture and dynamics of the
nuclear envelope. In contrast to other comprehensive works on
cellular membranes in general, this book is the first to
specifically address the nuclear envelope. Experts in the field
relate the different domains of the nuclear envelope, their
biogenesis, their composition, their mode of targeting to
chromosomes and how they dissociate from chromosomes upon cell
division. A wealth of information from several experimental systems
is provided, such as dividing somatic cells, embryos and nuclear
assembly assays in cell-free extracts, in a wide range of
vertebrate and invertebrate organisms ranging from humans to yeast.
The book also contains up-to-date reviews on the relationship
between mutations in proteins of the nuclear envelope and
Emery-Dreifuss muscular dystrophy. The breadth of topics covered in
what may seem to be a field as narrow as the nuclear envelope makes
this volume a valuable reference.
This volume provides a wide range of protocols used in studying the
nuclear envelope, with special attention to the experimental
adjustments that may be required to successfully investigate this
complex organelle in cells from various organisms. The Nuclear
Envelope: Methods and Protocols is divided into five sections: Part
I - Nuclear Envelope Isolation; Part II - Nuclear Envelope Protein
Interactions, Localization, and Dynamics; Part III - Nuclear
Envelope Interactions with the Cytoskeleton; Part IV - Nuclear
Envelope-Chromatin Interactions; and Part V - Nucleo-Cytoplasmic
Transport. Many of the modifications discussed in this book have
only been circulated within laboratories that have conducted
research in this field for many years. Written in the highly
successful Methods in Molecular Biology series format, chapters
include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Cutting edge and thorough, The Nuclear
Envelope: Methods and Protocols is a timely resource for
researchers who have joined this dynamic and rapidly growing field.
This volume provides an exhaustive review of the most current
knowledge of the composition, architecture and dynamics of the
nuclear envelope. In contrast to other comprehensive works on
cellular membranes in general, this book is the first to
specifically address the nuclear envelope. Experts in the field
relate the different domains of the nuclear envelope, their
biogenesis, their composition, their mode of targeting to
chromosomes and how they dissociate from chromosomes upon cell
division. A wealth of information from several experimental systems
is provided, such as dividing somatic cells, embryos and nuclear
assembly assays in cell-free extracts, in a wide range of
vertebrate and invertebrate organisms ranging from humans to yeast.
The book also contains up-to-date reviews on the relationship
between mutations in proteins of the nuclear envelope and
Emery-Dreifuss muscular dystrophy. The breadth of topics covered in
what may seem to be a field as narrow as the nuclear envelope makes
this volume a valuable reference.
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