This collection features five peer-reviewed literature reviews on using crops as biofuel. The first chapter reviews the key issues and challenges faced when expanding sugarcane cultivation for bioenergy, primarily, competition with food crops, loss of biodiversity, water, air and soil pollution, as well as a potential increase in pest problems. The second chapter describes current technologies and constraints of biodiesel production, including the need for a consistent supply of feedstock to ensure a sustainable biodiesel industry. The chapter also examines life cycle assessment (LCA) of canola/rapeseed biodiesel production. The third chapter addresses the sustainability of biofuels derived from palm oil and discusses the impact of their production on our ecosystems. The chapter also reviews the economics of biofuel and the levels of governmental support needed for sustainable production. The fourth chapter examines the potential of varying grassland types for biomass production. It details the options for use of grassland biomass, citing its suitability for combustion, pyrolysis, biorefining and for use in bioenergy applications. The final chapter reviews best agronomic practices for increasing overall biomass output in the face of pressing environmental changes. It also utilises the LCA framework to review strategies for crop improvement, such as crop rotations.