This open access book constitutes the refereed proceedings of the 16th International Annual Conference on Cyber Security, CNCERT 2020, held in Beijing, China, in August 2020. The 17 papers presented were carefully reviewed and selected from 58 submissions. The papers are organized according to the following topical sections: access control; cryptography; denial-of-service attacks; hardware security implementation; intrusion/anomaly detection and malware mitigation; social network security and privacy; systems security.