This volume is a compilation of sixteen chapters that detail reverse genetics protocols. Reverse Genetics of RNA Viruses: Methods and Protocols guides readers through comprehensive protocols on RNA viruses, that were the most challenging to obtain and/or that were developed most recently. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Reverse Genetics of RNA Viruses: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Bioarchaeology is the analysis of human remains within an interpretative framework, including a wide range of contextual information. This comprehensive and much-needed manual provides both a starting point and a reference for archaeologists working in this integrative field. The authors cover a range of bioarchaeological methods and theory including: * Ethical issues involved in dealing with human remains, specifically related to NAGPRA * Field and taphonomic clues * Lab and Forensic techniques * Best practices methods for Excavation techniques * Special applications of Bioarchaeology * Theoretical frameworks of Bioarchaeology With case studies from over twenty years each of bioarchaeological research, the authors integrate theoretical and methodological discussion with a wide range of field studies, from different geographic areas, time periods, and data types, to demonstrate the full scope of this important field of study.

This is the first inter-group and gender inclusive collection of scholarship in U.S. Latino literary criticism that begins with the assumption that the literature written by U.S. Latinos is as important an object of scholarship as U.S. Latino/a history, sociology, and culture, fields that have dominated previous inter-group anthologies. Some of the most important and insightful Latino and Latina literary scholars in the field write on authors from the four major Latino/a groups-- Cuban American, Dominican American, Mexican American, and Puerto Rican American. The anthology evaluates the state of U.S. Latino/a literary study and projects a vision of that study for the twenty-first century. This book is divided into four major areas of literary inquiry: analyses of the psychic relations between the Latino/a subject and its mimetic others; explorations of the complexities of race and Afro-Latino/a poetics; studies of the representation of labor in the Latino/a literary imagination; and genealogical and archival assessment of U.S. Latino literature's relationship with American, Caribbean, and Latin American literatures and histories.

"Long Wave Polar Modes in Semiconductor Heterostructures" is concerned with the study of polar optical modes in semiconductor heterostructures from a phenomenological approach and aims to simplify the model of lattice dynamics calculations. The book provides useful tools for performing calculations relevant to anyone who might be interested in practical applications.

The main focus of "Long Wave Polar Modes in Semiconductor Heterostructures" is planar heterostructures (quantum wells or barriers, superlattices, double barrier structures etc) but there is also discussion on the growing field of quantum wires and dots. Also to allow anyone reading the book to apply the techniques discussed for planar heterostructures, the scope has been widened to include cylindrical and spherical geometries.

The book is intended as an introductory text which guides the reader through basic questions and expands to cover state-of-the-art professional topics. The book is relevant to experimentalists wanting an instructive presentation of a simple phenomenological model and theoretical tools to work with and also to young theoreticians by providing discussion of basic issues and the basis of advanced theoretical formulations. The book also provides a brief respite on the physics of piezoelectric waves as a coupling to polar optical modes.

A collection of essays that explores magical realism as a momentary interruption of realism in US ethnic literature, showing how these moments of magic realism serve to memorialize, address, and redress traumatic ethnic histories.

All of a sudden, it hit me: while I am waiting on the Lord to work in my life; the Lord is waiting on me. This was the moment in time for me to share His message of love for us, through my own personal journey. I've stepped out in faith to share with others of his amazing grace. Thank you, Father God all glory, honor and praise, I give onto your precious holy name. I'm getting it I'm sitting here with a great big smile.

I don't know how the story of my life is going to end, but I know the one that does. On that note, I send blessings to all the women of faith, along with those who are and will become pillars of strength for their families. God is ever so faithful, and he does have a sense of humor; see how he, our heavenly Father, works all things out for good for those who believe. Be still and know that God is still God; He is mighty in working miracles and wonders He is still our healer, and He wants to show Himself strong on behalf of those who trust in Him. Keep believing and never give up.

While reading, you will be drawn into a life journey filled with lots of love and laughter, as well as tragic losses. You will find golden nuggets of wisdom, faith, courage, loss and victory sprinkled throughout. My Journey My Destiny, "He wrapped Me in His Light" is a source of encouragement, not only to single mothers, but to all women who have struggled with the loss of a loved one, have had health issues, been unemployed...but never lost hope, and how you can become an unshakeable women of faith

This book provides an in-depth exploration of scientific photography. Highlighting the best practices needed to make, distribute, and preserve scientific visual information using digital photographic methods and technologies, it offers solutions to some of the biggest challenges facing photographers. Written by a team of international, award-winning image makers with over 300 years of cumulative experience, this comprehensive resource explains the foundations used, the tools required, and the steps to needed for creating the optimal photograph in a range of environments and circumstances. Topics covered include: * ethical practices * aerial photography * close-up and macro photography * computational photography * field photography * geological photography * imaging with invisible spectrums * photographing small animals in captivity * time-based imaging * image processing in science Showcasing modern methods, this book equips readers with the skills needed to capture and process the best image possible. Designed for basic and intermediate photographers, Natural Science Imaging and Photography exists as an essential contemporary handbook.

This book provides an in-depth exploration of scientific photography. Highlighting the best practices needed to make, distribute, and preserve scientific visual information using digital photographic methods and technologies, it offers solutions to some of the biggest challenges facing photographers. Written by a team of international, award-winning image makers with over 300 years of cumulative experience, this comprehensive resource explains the foundations used, the tools required, and the steps to needed for creating the optimal photograph in a range of environments and circumstances. Topics covered include: * ethical practices * aerial photography * close-up and macro photography * computational photography * field photography * geological photography * imaging with invisible spectrums * photographing small animals in captivity * time-based imaging * image processing in science Showcasing modern methods, this book equips readers with the skills needed to capture and process the best image possible. Designed for basic and intermediate photographers, Natural Science Imaging and Photography exists as an essential contemporary handbook.

This volume is a compilation of sixteen chapters that detail reverse genetics protocols. Reverse Genetics of RNA Viruses: Methods and Protocols guides readers through comprehensive protocols on RNA viruses, that were the most challenging to obtain and/or that were developed most recently. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Reverse Genetics of RNA Viruses: Methods and Protocols aims to ensure successful results in the further study of this vital field.

vi The intent, therefore, was to provide for a fresh and original review of all relevant topics and issues in the field, following a comprehensive and coherent programme. Such an ambitious goal could only be reached thanks to the unlimited collaboration of the lecturers: They were requested to produce nothing less than "freer, broader, speculative and personal "considerations of the subjects" they had to cover *** And so they did: their presentations unfolded a fantastic picture, a most fascinating and meaningful identification of the field, its present problems and trends. But participants at this conference contributed many valuable observations while discussing specific points. Unfortunately, more often than not, it proved impossible to identify them in the records. Accordingly, we incorporated here and there some of these "anonymous" contributions as part of the edited version of the texts. To all of the participants, I would like to express my gratitude for their actively taking part in all the scientific (and social *** ) activities (lectures, round tables, posters, encounters) of this N.A.T.O. ASl. The result was a stimulating atmosphere, conducive to authentic scientific exchanges. Hence, the success of this meeting should be credited only to them. Finally, on behalf of all participants, I would like to thank Professor Sissini, the Mayor of Maratea, and Mr. B. Vitolo, the Chairman of its Tourist Office: their enthusiastic support greatly contributed to make of our time in their wonderful seaside resort an unforgettable one.

A collection of essays that explores magical realism as a momentary interruption of realism in US ethnic literature, showing how these moments of magic realism serve to memorialize, address, and redress traumatic ethnic histories.

vi The word ppotein, coined one and a half century ago from the 1TpOTE:toa ("proteios" = of primary importance), underlines the "primary importance" ascribed to proteins from the time they were described as biochemical entities. But the unmatched compl~xity of the process involved in their biosynthesis was (understandably) overlooked. Indeed, protein biosynthesis was supposed to be nothing more than the reverse of protein degradation, and the same enzymes known to split a protein into its constituent amino acids were thought to be able, under adequate conditions, to reconstitute the peptide bond. This oversimplified view persisted for more than 50 years: It was just in 1940 that Borsook and Dubnoff examined the thermodynamical aspects of the process, and concluded that protein synthesis could not be the reverse of protein degradation, such an "uphill task being thermody- namically impossible *** * " The next quarter of a century witnessed the unravelling of the basic mechanisms of protein biosynthesis, a predictable aftermath of the Copernican revolution in biology which followed such dramatic de- velopments as the discovery of the nature of the genetic material, the double helical structure* of DNA, and the determination of the ge- netic code. Our present understanding of the sophisticated mechan- isms of regulation and control is a relatively novel acquisition, and recent studies have shed some light into the structure and organi- zation of the eukaryotic gene.

Please note this is a 'Palgrave to Order' title (PTO). Stock of this book requires shipment from an overseas supplier. It will be delivered to you within 12 weeks. This is the first compilation of essays to bring together the most important U.S. Latino/a literary criticism of the last decade. This timely text has been long in coming as U.S. Latino/a literary criticism has grown exponentially throughout U.S universities since 1995.