This book serves as a tool to expand the knowledge of researchers in the discipline of microbiology and biochemistry encompassing the fields of molecular biology, structural biology, and crystallography for the two very important industrial enzymes: proteases and lipases. In addition it covers the fermentation technology for the production of locally isolated microbial lipases and proteases. This book presents work done in our laboratory which extensively reviews two major types of lipases and proteases: thermostable and/or organic-solvent tolerant including a chapter on cold-active and organic solvent tolerant lipase and protease isolated from Antarctica. Detailed descriptions on the isolation methods, protein production, protein characterisation, and molecular cloning are discussed. Further works on structural biology of both proteases and lipases which include docking, molecular modification as well as protein crystallisation are also presented. For protein crystallographers, this book highlights a novel discovery of high temperature crystallisation of lipase. In addition, this book also offers a chapter on the cultivation strategy of lipase and protease in yeast expression systems and its bio-reactor studies. In short, this book is an all-in-one quick source of reference for researchers who want to have a quick grasp of understanding on many aspects of enzyme production, starting from the isolation and expression of the enzyme until crystallisation and structure elucidation of the protein.

Enzymatic processing of lipids and oils is becoming an important area of research. Hydrolytic enzymes, such as lipases and proteases are being sought after as the biocatalysts of the future. Synthetic reactions to create new compounds that have novel properties may be achieved through biocatalysis. Some of these compounds can improve or even replace on existing products that were produced by inorganic catalysis or being fractionated from un-renewable resources, such as petroleum. The authors focused on lipases and proteases as the most critical enzymes. Proteases are already widely used in industry and there is always the possibility for new enzymes to be used in existing and new applications. Lipases have an exciting potential for advancing the bioprocessing industry, in particular oleochemicals. Thermostable enzymes are always sought by the industries. Whereas solvent tolerant enzymes are becoming the vogue in view of their ability to function in a low aqueous medium, suitable for synthetic reactions. The research in this book involves a broad range of the biotechnology scope. However, the focus of this book is on the search and acquisition, isolation and purification and the characterisation of these enzymes. However, derivatised enzymes must also be taken into consideration. The authors' laboratory is involved in the development and application of immobilised and modified enzymes. These designer enzymes may prove to be the new enzymes that would really expand the potential applications of enzymes in industries.