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Unlike some other reproductions of classic texts (1) We have not
used OCR(Optical Character Recognition), as this leads to bad
quality books with introduced typos. (2) In books where there are
images such as portraits, maps, sketches etc We have endeavoured to
keep the quality of these images, so they represent accurately the
original artefact. Although occasionally there may be certain
imperfections with these old texts, we feel they deserve to be made
available for future generations to enjoy.
Unlike some other reproductions of classic texts (1) We have not
used OCR(Optical Character Recognition), as this leads to bad
quality books with introduced typos. (2) In books where there are
images such as portraits, maps, sketches etc We have endeavoured to
keep the quality of these images, so they represent accurately the
original artefact. Although occasionally there may be certain
imperfections with these old texts, we feel they deserve to be made
available for future generations to enjoy.
Thin-layer chromatography (TLC) has become a common and much
favoured separation technique in laboratories in widely varied
fields in recent years. Much of the credit for the introduction of
this technique into analytical practice at the l 2 end of the 1950s
is due to E. Stahl * * This method is simple and is characterized
by high separation ability and sufficient sensitivity3; however,
some analysts feel that it has passed the peak in its development
and will gradually be replaced by the more modem high-performance
liquid chromatography (HPLC). This is undoubtedly a very important
analytical technique utilizing the specific separa tion properties
of a large number of sorbents and the possibility of regulating 4
the flow-rate of the mobile phase by adjusting the pressure *
Standardization of the experimental conditions is simpler in HPLC
than in TLC, where the activity of the sorbent and flow-rate of the
eitlent in the thin layer depend markedly on the relative humidity
of the laboratory atmosphere and on the composition of the gaseous
phase in the elution chamber. In addition, systems for quantitative
detection of the separated ~ones are better developed for HPLC than
for classical TLC, where, until recently, cumbersome and often even
insufficiently reproducible chemical or gravimetric analysis of the
extracts of scraped-off spots or densitometry of the separated
zones, located first by pyrolysis or reaction s with suitable
detection agents, were the predominant determination methods .
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