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A novel, rapid and cost-effective chlorpromazine hydrochloride
(CPZH) decolorization assay is described for the screening of
antioxidant activity. A chromogenic reaction between CPZH and
potassium persulfate at low pH produces an reddish-pink radical
cation with maximum absorption at 527 nm in its first-order
derivative spectrum. The antioxidant capacity of standard solutions
of an antioxidant was evaluated by comparing with the inhibition
curve using Trolox as the standard. Comparison of antioxidant
capacity determined with this newly developed CPZH assay and with
the well-known 2,2'- azinobis- 3-ethylbenzthiazoline-6-sulfonic
acid] (ABTS)-persulfate decolorization assay indicated the efficacy
and sensitivity of the procedure.This method is applied on the
apple juices of different brands and on Canola seeds of Different
cultivars In this study total antioxidant capacity (TAC) of
extracts of Canola seeds of different cultivars Leaves Zafar 2000
(Z1, Z2)Bulbul 98 (B1, B2) and Pakola (P1, P2) was investigated.
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