This volume bridges the gap between the global promotion of the Green Economy and the manifestation of this new development strategy at the urban level. Green cities are an imperative solution, not only in meeting global environmental challenges but also in helping to ensure socio-economic prosperity at the local level.

This volume bridges the gap between the global promotion of the Green Economy and the manifestation of this new development strategy at the urban level. Green cities are an imperative solution, not only in meeting global environmental challenges but also in helping to ensure socio-economic prosperity at the local level.

This third volume provides comprehensive protocols on pre-analytical, analytical, plasma, and serum proteomics. New and updated chapters are divided into nine sections, detailing blood processing and handling strategies, discovery- and targeted-based mass spectrometry, including workflows to aid in discovery and targeted data analysis, in addition to software and bioinformatics for the plasma proteome. This edition further integrates emerging areas in the development of technologies for plasma proteomics and assay platforms in biomarker discovery and translational proteomics, enrichment and detection strategies to understand the plasma proteome, and peptide, lipid and metabolite targeted assays. We also detail the emerging analysis of extracellular vesicles isolated from plasma. Written in the format of the highly successful Methods in Molecular Biology series, each of the 33 chapters includes an introduction to the topic, lists necessary materials and methods, includes hints and tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Serum/Plasma Proteomics: Methods and Protocols, Third Edition aims to be comprehensive guide for researchers.

This is the complete history of a building that began as a hunting lodge, late in the eleventh century and that grew to be the principal house of the manor of Theydon Mount in Essex, a small country retreat within easy reach of London. In 1556, the house was acquired by Sir Thomas Smith (1512-77), a man of humble origins but precocious intellect who became Regius Professor of Civil Law at Cambridge at the age of thirty and Chancellor of the University two years later. He then forsook academic for political life, becoming Master of Requests to the Lord Protector Somerset. From 1557, Smith rebuilt the house in French-influenced classical style and decorated it with wall paintings of Cupid and Psyche and King Hezekiah, conveying complex messages of morality and affinity as part of a coherent programme of images in paint, glass and tiles. Four centuries on, the house was first used as an open prison, then, in 1969, largely gutted by fire and finally, in 1980, taken into the care of the Department of the Environment. Archaeological excavation and detailed recording of the surviving fabric took place prior to the restoration of the house and its mural paintings, the results of which are now presented in this copiously illustrated account of one of the most important and influential houses to be built in Elizabethan England.