This second edition volume brings together the experiences of leading scientists in the discipline of cancer gene profiling. Because cancer genes can be profiled in many different ways, Cancer Gene Profiling: Methods and Protocols, Second Edition explores different techniques and approaches needed to understand the key stages of cancer development, as using only one technique would be insufficient. This book provides readers with an overview of the state-of-the-art methods that will enable them to perform these experiments, and demonstrates the kind of analysis that is possible in our current biomedical research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Cancer Gene Profiling: Methods and Protocols, Second Edition is a great resource for any student or practitioner with an interest in cancer gene profiling, and can be used in any well-equipped research laboratory.

This second edition volume brings together the experiences of leading scientists in the discipline of cancer gene profiling. Because cancer genes can be profiled in many different ways, Cancer Gene Profiling: Methods and Protocols, Second Edition explores different techniques and approaches needed to understand the key stages of cancer development, as using only one technique would be insufficient. This book provides readers with an overview of the state-of-the-art methods that will enable them to perform these experiments, and demonstrates the kind of analysis that is possible in our current biomedical research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Cancer Gene Profiling: Methods and Protocols, Second Edition is a great resource for any student or practitioner with an interest in cancer gene profiling, and can be used in any well-equipped research laboratory.

Cystic tumours of the pancreas today are diagnosed more frequently in clinical practice, mainly due to an increased use of the modern advanced imaging modalities. Bland cysts of the pancreas most often develop after chronic or acute inflammation of the pancreas. However, the current knowledge concerning the development of cystic neoplasias of the pancreas is still rudimentary. Histopathologically, 90% of pancreatic cystic neoplasias are represented by four types: serous microcystic (SCN), mucinous cystic (MCN), intraductal papillary mucinous (IPMN) and solid pseudopapillary (SPN) neoplasias. Surgical treatment of these lesions can be highly challenging and occasionally demands complex surgical approaches that should be put in the hands of skilled pancreatic surgeons in experienced high-volume centres. While some of the described cystic tumours are harmless, such as SCNs of the pancreas, others such as IPMN and MCN harbour relevant malignant potential. The differential diagnosis of these lesions is critical for the patient- but often difficult. In addition, surgical treatment, even in the hands of skilled surgeons in centres of pancreatic surgery, can be complicated and can result in relevant morbidity and mortality. Therefore, it is of great importance for all doctors treating and advising patients with cystic tumours of the pancreas, i.e. surgeons, gastroenterologists, radiologists and pathologists as well as for the general practitioner, to have funded knowledge in regard to epidemiology, pathology, diagnostics and prognosis of the various pancreatic lesions, regardless of the necessity for surgical treatment. Together with recognised specialists in this field from Europe, the United States, and Japan, the authors offer a broad and detailed overview over diagnosis and the current treatment strategies for patients with cystic tumours of the pancreas.