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Multipotent Stem Cells of the Hair Follicle - Methods and Protocols (Hardcover, 1st ed. 2016): Robert M. Hoffman Multipotent Stem Cells of the Hair Follicle - Methods and Protocols (Hardcover, 1st ed. 2016)
Robert M. Hoffman
R4,053 R3,331 Discovery Miles 33 310 Save R722 (18%) Ships in 12 - 17 working days

This volume discusses methods for the study of multipotent and pluripotent stem cells of the hair follicle. The stem cells described are involved in both the growth of the hair follicle and its production of the hair shaft, as well as the growth of the hair follicle sensory nerve. Multipotent Stem Cells of the Hair Follicle: Methods and Protocols also explores very unexpected results such as that of the hair follicle-associated-pluripotent (HAP) stem cells, which not only have the capability for regenerating the hair follicle sensory nerve, but also can differentiate ex vivo and in vivo to multiple cell types not associated with the hair follicle-these include glial cells, motor neurons, and beating cardiac muscle cells. The potential for HAP stem cells for regenerative medicine is also discussed in detail. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Multipotent Stem Cells of the Hair Follicle: Methods and Protocols is a valuable resource for researchers interested in this field.

3D Sponge-Matrix Histoculture - Methods and Protocols (Hardcover, 1st ed. 2018): Robert M. Hoffman 3D Sponge-Matrix Histoculture - Methods and Protocols (Hardcover, 1st ed. 2018)
Robert M. Hoffman
R1,519 Discovery Miles 15 190 Ships in 10 - 15 working days

This volume describes numerous applications of sponge-matrix histoculture to study cancer biology and the treatment of cancer, stem cells, organoids, growth and repair of nerves, lymphoid tissues that produce antibodies, and HIV infection. The chapters in this book cover topics including, in vivo-like growth patterns of multiple types of tumors in Gelfoam (R) histoculture; development of the histoculture drug response assay (HDRA) for cancer patients; hair-shaft growth in Gelfoam (R) histoculture of skin as well as isolated hair follicles in Gelfoam (R) histoculture; the use of Gelfoam (R) histoculture to discover novel treatment of HIV infection; and imaging DNA repair after UV irradiation damage of cancer cells in Gelfoam (R) histoculture. The volume explains what is true 3D cell and tissue culture and corrects widespread misconceptions of 3D culture in the literature. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, 3D Sponge-Matrix Histoculture: Methods and Protocols is a valuable resource that contributes to new cell and tissue culture research, and other diseases based on the true 3D culture.

Mouse Genetics - Methods and Protocols (Hardcover, 2nd ed. 2021): Shree Ram Singh, Robert M. Hoffman, Amit Singh Mouse Genetics - Methods and Protocols (Hardcover, 2nd ed. 2021)
Shree Ram Singh, Robert M. Hoffman, Amit Singh
R4,985 Discovery Miles 49 850 Ships in 10 - 15 working days

This fully updated edition provides selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish the manipulation of genes of interest, tracing cell lineages, and modeling human diseases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Mouse Genetics: Methods and Protocols, Second Edition delivers fundamental techniques and protocols to geneticists, molecular biologists, cell and developmental biologists, students, and postdoctoral fellows working in the various disciplines of genetics, developmental biology, mouse genetics, and modeling human diseases.

Multipotent Stem Cells of the Hair Follicle - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed.... Multipotent Stem Cells of the Hair Follicle - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2016)
Robert M. Hoffman
R3,646 Discovery Miles 36 460 Ships in 10 - 15 working days

This volume discusses methods for the study of multipotent and pluripotent stem cells of the hair follicle. The stem cells described are involved in both the growth of the hair follicle and its production of the hair shaft, as well as the growth of the hair follicle sensory nerve. Multipotent Stem Cells of the Hair Follicle: Methods and Protocols also explores very unexpected results such as that of the hair follicle-associated-pluripotent (HAP) stem cells, which not only have the capability for regenerating the hair follicle sensory nerve, but also can differentiate ex vivo and in vivo to multiple cell types not associated with the hair follicle-these include glial cells, motor neurons, and beating cardiac muscle cells. The potential for HAP stem cells for regenerative medicine is also discussed in detail. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Multipotent Stem Cells of the Hair Follicle: Methods and Protocols is a valuable resource for researchers interested in this field.

Mouse Genetics - Methods and Protocols (Paperback, 2nd ed. 2021): Shree Ram Singh, Robert M. Hoffman, Amit Singh Mouse Genetics - Methods and Protocols (Paperback, 2nd ed. 2021)
Shree Ram Singh, Robert M. Hoffman, Amit Singh
R3,267 Discovery Miles 32 670 Ships in 10 - 15 working days

This fully updated edition provides selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish the manipulation of genes of interest, tracing cell lineages, and modeling human diseases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Mouse Genetics: Methods and Protocols, Second Edition delivers fundamental techniques and protocols to geneticists, molecular biologists, cell and developmental biologists, students, and postdoctoral fellows working in the various disciplines of genetics, developmental biology, mouse genetics, and modeling human diseases.

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