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Over the last decade, interest in mycoplasmas has been greatly sti-
lated by the spread of contagious bovine pleuropneumonia (CBPP),
caused by Mycoplasma mycoides subsp. mycoides SC, in Europe and
Africa and the discovery of a possible association between
mycoplasmas and AIDS. During this period there has also been a
recognition by control authorities and int- national organizations,
including the Office International des Epizooties, that mycoplasmas
are major causes of economic loss in livestock and continue to be a
problem in humans as a result of a range of chronic respiratory and
urogenital diseases. However, it is likely that the true
significance of my- plasmas is still underestimated because of the
difficulties in working with these extremely fastidious
microorganisms. Advances in such laboratory technology as DNA
amplification has enabled the detection of unrecoverable
mycoplasmas in tissue samples and this will provide us with a
better understanding of their role in disease. Important
developments have also been seen in more conv- tional areas such as
serological tests and media formulation enabling improved recovery
and identification. Mycoplasma Protocols provides in a single
volume up-to-date and easy-- follow methods for the detection,
isolation, identification, and characterization of mycoplasmas,
with a major emphasis on those of medical and veterinary
significance. It also includes biochemical, genetic, and molecular
techniques that will form the basis of understanding pathogenicity
and, particularly, - hesion to host cells.
Over the last decade, interest in mycoplasmas has been greatly sti-
lated by the spread of contagious bovine pleuropneumonia (CBPP),
caused by Mycoplasma mycoides subsp. mycoides SC, in Europe and
Africa and the discovery of a possible association between
mycoplasmas and AIDS. During this period there has also been a
recognition by control authorities and int- national organizations,
including the Office International des Epizooties, that mycoplasmas
are major causes of economic loss in livestock and continue to be a
problem in humans as a result of a range of chronic respiratory and
urogenital diseases. However, it is likely that the true
significance of my- plasmas is still underestimated because of the
difficulties in working with these extremely fastidious
microorganisms. Advances in such laboratory technology as DNA
amplification has enabled the detection of unrecoverable
mycoplasmas in tissue samples and this will provide us with a
better understanding of their role in disease. Important
developments have also been seen in more conv- tional areas such as
serological tests and media formulation enabling improved recovery
and identification. Mycoplasma Protocols provides in a single
volume up-to-date and easy-- follow methods for the detection,
isolation, identification, and characterization of mycoplasmas,
with a major emphasis on those of medical and veterinary
significance. It also includes biochemical, genetic, and molecular
techniques that will form the basis of understanding pathogenicity
and, particularly, - hesion to host cells.
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