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An efficient in vitro plant regeneration and Agrobacterium
tumefaciens mediated genetic transformation protocol was described
for castor (Ricinus communis L.) using meristem as explants. Shoot
apex containing apical meristems were excised from 5-7 days old in
vitro grown seedlings and cultured on Murashige and Skoog (MS)
medium supplemented with different concentrations of cytokinins
singly or in combination. Kinetin (0.50 mg/l) in combination with
BAP (0.25 mg/l) produced maximum number of shoot (10.33) and shoot
length (5.20 cm). For root induction, in vitro produced shoots were
transferred to rooting media containing 1/2 MS basal media with
NAA. In Agrobacterium tumefaciens mediated genetic transformation,
Agrobacterium tumefaciens strain containing construct pBIN1F
harboring nptII and cry1F gene was used. The integration of the
gene was confirmed by using PCR. The transformation experiment was
performed by optimizing age of the seedlings and co-cultivation
duration with Agrobacterium. When the effect of the age of the
seedlings and co-cultivation duration evaluated fifteen days old
seedlings and co-cultivated for three days yielded the frequency of
transformation 2
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