There is an increasing interest of biotechnologists in the potential of cold-adapted organisms, since they play a major role in the processes of nutrient turnover and primary biomass production in cold ecosystems. Essential advantages of the application of such organisms are the rapid microbial metabolism at low temperatures, the low activation energy for enzymatic substrate hydrolysis and the low thermostability of enzymes from cold-adapted organisms. Benefits can also be deduced from the frost hardiness and frost resistance of cold-adapted plants and animals.

Yeasts are a versatile group of eukaryotic microorganisms, exhibiting heterogeneous nutritional profiles and an extraordinary ability to survive in a wide range of natural and man-associated ecosystems, including cold habitats. Cold-adapted yeasts inhabit numerous low-temperature environments where they are subjected to seasonal or permanent cold conditions. Hence, they have evolved a number of adaptation strategies with regard to growth and reproduction, metabolic activities, survival and protection. Due to their distinctive ability to thrive successfully at low and even subzero temperatures, cold-adapted yeasts are increasingly attracting attention in basic science and industry for their enormous biotechnological potential.

This book presents our current understanding of the diversity and ecology of cold-adapted yeasts in worldwide cold ecosystems, their adaptation strategies, and their biotechnological significance. Special emphasis is placed on the exploitation of cold-adapted yeasts as a source of cold-active enzymes and biopolymers, as well as their benefits for food microbiology, bioremediation and biocontrol. Further, aspects of food biodeterioration are considered.

Most of the Earth's biosphere is characterized by low temperatures. Vast areas (>20%) of the soil ecosystem are permanently frozen or are unfrozen for only a few weeks in summer. Permafrost regions occur at high latitudes and also at high ele- tions; a significant part of the global permafrost area is represented by mountains. Permafrost soils are of global interest, since a significant increase in temperature is predicted for polar regions. Global warming will have a great impact on these soils, especially in northern regions, since they contain large amounts of organic carbon and act as carbon sinks, and a temperature increase will result in a release of carbon into the atmosphere. Additionally, the intensified release of the clima- relevant tracer gas methane represents a potential environmental harzard. Significant numbers of viable microorganisms, including bacteria, archaea, p- totrophic cyanobacteria and green algae, fungi and protozoa, are present in per- frost, and the characteristics of these microorganisms reflect the unique and extreme conditions of the permafrost environment. Remarkably, these microorg- isms have been reported to be metabolically active at subzero temperatures, even down to ?20 DegreesC.

Representing the latest knowledge of the ecology and the physiology of cold-adapted microorganisms, plants and animals, this book explains the mechanisms of cold-adaptation on the enzymatic and molecular level, including results from the first crystal structures of enzymes of cold-adapted organisms.

Reliable methods for monitoring and assessing soil quality are a prerequisite for successful soil bioremediation projects. The fifth volume of Soil Biology presents detailed descriptions of selected methods for evaluating, monitoring and assessing bioremediation treatments of soils contaminated with organic pollutants or heavy metals.

Traditional soil investigation techniques, including chemical, physical and microbiological methods, are complemented by the most suitable modern methods, such as the use of bioreporter technology, immunological, ecotoxicological or molecular assays. Feasibility studies for bioremediation treatments complete the manual. Easy-to-follow protocols with step-by-step procedures, lists of the required equipment and reagents as well as notes on the evaluation and quality control allow immediate application. Short introductions to the principles and objectives help to assess the field of application of each procedure.

Yeasts are a versatile group of eukaryotic microorganisms, exhibiting heterogeneous nutritional profiles and an extraordinary ability to survive in a wide range of natural and man-associated ecosystems, including cold habitats. Cold-adapted yeasts inhabit numerous low-temperature environments where they are subjected to seasonal or permanent cold conditions. Hence, they have evolved a number of adaptation strategies with regard to growth and reproduction, metabolic activities, survival and protection. Due to their distinctive ability to thrive successfully at low and even subzero temperatures, cold-adapted yeasts are increasingly attracting attention in basic science and industry for their enormous biotechnological potential. This book presents our current understanding of the diversity and ecology of cold-adapted yeasts in worldwide cold ecosystems, their adaptation strategies, and their biotechnological significance. Special emphasis is placed on the exploitation of cold-adapted yeasts as a source of cold-active enzymes and biopolymers, as well as their benefits for food microbiology, bioremediation and biocontrol. Further, aspects of food biodeterioration are considered.

How can industry profit from the biochemical tricks of cold-adapted organisms? This book covers a range of aspects in this fascinating field, from genetic tools to environmental biotechnology.

In terrestrial ecosystems, soil microorganisms and soil animals are essential for litter degradation, soil formation and the availability of nutrients and trace elements. The measurement of biological soil parameters allows a rapid evaluation of the effects of chemical and physical influences due to pollutants or soil management.
This book introduces a number of well proved methods for the analysis of carbon, nitrogen, phosphorus and sulfur cycles. It focuses further on the determination of the number and biomass of microorganisms, algae and animals in the soil. Particular emphasis is placed on the comprehensible and complete description of the experimental procedures.

Reliable methods for monitoring and assessing soil quality are a prerequisite for successful soil bioremediation projects. The fifth volume of Soil Biology presents detailed descriptions of selected methods for evaluating, monitoring and assessing bioremediation treatments of soils contaminated with organic pollutants or heavy metals.

Traditional soil investigation techniques, including chemical, physical and microbiological methods, are complemented by the most suitable modern methods, such as the use of bioreporter technology, immunological, ecotoxicological or molecular assays. Feasibility studies for bioremediation treatments complete the manual. Easy-to-follow protocols with step-by-step procedures, lists of the required equipment and reagents as well as notes on the evaluation and quality control allow immediate application. Short introductions to the principles and objectives help to assess the field of application of each procedure.

Die Messung biologischer Parameter von Boden erlaubt eine rasche Beurteilung der Auswirkung chemischer und physikalischer Einflusse auf den Boden. Dieses Buch stellt eine Vielzahl von enzymatischen Methoden vor, die zur Erfassung von Aktivitaten aus dem Kohlenstoff-, Stickstoff-, Phosphor- und Schwefelkreislauf geeignet sind. Ebenso wird die Bestimmung der Biomasse von Mikroorganismen, Algen und Tieren des Bodens beschrieben. Das Methodenspektrum wurde gegenuber der ersten Auflage um einen zoologischen Teil und zahlreiche aktuelle mikrobiologische Methoden erweitert. Bei den Arbeitsvorschriften wurde besonders auf eine verstandliche und vollstandige Beschreibung der experimentellen Ablaufe geachtet.

Altlasten und rezente Kontaminationen durch Unfalle und menschliches Fehlverhalten stellen eine Gefahrdung der Biosphare dar. Zum Schutz von Mensch, Tier und Pflanze mussen Boden, Wasser und Luft von anorganischen und organischen Schadstoffen moglichst freigehalten werden. Fur die Sanierung von Boden stehen dabei unterschiedliche Technologien zur Verfugung. Die Vorteile einer mikrobiologischen Bodensanierung liegen in einer hohen Umweltvertraglichkeit des Verfahrens, das durch minimalen Energieeinsatz und ohne Schaffung neuer Entsorgungsprobleme die Elimination und auch Mineralisation der Schadstoffe ermoglicht.