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Of all scientific instruments, probably none has had more
applications in the life sciences than the light microscope. In
Light Microscopy: Methods and Protocols, expert researchers explore
the basics and the latest advances in microscope instrumentation,
sample preparation, and imaging techniques, all of which have been
producing fundamental insights into the functions of cells and
tissues. Chapters cover a variety of bright field and fluorescence
microscopy-based approaches that are central to the study of a
range of biological questions, providing information on how to
prepare cells and tissues for microscopic investigations, covering
detailed staining procedures, and exploring methods to analyze
images and interpret the results accurately. Composed in the highly
successful Methods in Molecular Biology (TM) series format, each
chapter contains a brief introduction, step-by-step methods, a list
of necessary materials, and a Notes section which shares tips on
troubleshooting and avoiding known pitfalls. Comprehensive and
current, Light Microscopy: Methods and Protocols is an essential
handbook for all researchers who are exploring the intriguing
microscopic world of the cell.
Of all scientific instruments, probably none has had more
applications in the life sciences than the light microscope. In
Light Microscopy: Methods and Protocols, expert researchers explore
the basics and the latest advances in microscope instrumentation,
sample preparation, and imaging techniques, all of which have been
producing fundamental insights into the functions of cells and
tissues. Chapters cover a variety of bright field and fluorescence
microscopy-based approaches that are central to the study of a
range of biological questions, providing information on how to
prepare cells and tissues for microscopic investigations, covering
detailed staining procedures, and exploring methods to analyze
images and interpret the results accurately. Composed in the highly
successful Methods in Molecular Biology(TM) series format, each
chapter contains a brief introduction, step-by-step methods, a list
of necessary materials, and a Notes section which shares tips on
troubleshooting and avoiding known pitfalls. Comprehensive and
current, Light Microscopy: Methods and Protocols is an essential
handbook for all researchers who are exploring the intriguing
microscopic world of the cell.
Eosinophil Ultrastructure: Atlas of Eosinophil Cell Biology and
Pathology entirely focuses on eosinophils and their functional
roles in inflammation, host defense, and normal homeostatic
activities. The book explores the ultrastructure of human
eosinophils, highlighting biological processes observed under
normal, experimental, and pathological conditions. Created to fill
a void in the eosinophil literature, the book includes an extensive
array of electron microscopic images that illustrate the diversity
of eosinophil morphology. While the atlas is a learning and
teaching tool, it is mainly a helpful resource for researchers to
identify distinguishing features and structural changes that arise
during studies of human eosinophils. The book also covers the
ultrastructure of mouse eosinophils under normal and activation
conditions and in the context of representative diseases.
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