In the past two decades, the importance of animal cell technology has increased enormously. First, useful proteins can be produced by cultured animal cells, in which the desired product can be modified and organized so as to retain its biological function. Second, studies of cultured cells can provide information needed to understand molecular mechanisms that govern what happens in tissues, organs, and even entire organisms. For this second purpose, biochemists and molecular biologists may need a large number of such cells. Third, cultured cells can be used instead of tissues and organs clinically. The Third Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), at which participants from abroad were warmly welcomed, was held in Kyoto on December 11-13, 1990. It was organized around the idea of providing a place for the review of much new data on such applications of cultured cells and for exchanges of the views of the participants about progress in the field. This volume, divided into seven sections, contains the proceedings of the meeting. The first section reviews the molecular basis of the control of animal cell growth. In the following sections, physicochemical and biochemical factors for cell growth and production of biologicals, cell culture systems including serum-free culture, new cell lines, specific products and their characteristics, and in vitro assays for toxic, carcinogenic, and pharmacological effects are taken up in their tum.

Animal cell technology is a growing discipline of cell biology which aims not only to understand structures, functions and behaviors of differentiated animal cells but also to ascertain their abilities to be used in industrial and medical purposes. The goal of animal cell technology includes accomplishments of clonal expansion of differentiated cells with useful ability, optimization of their culture conditions, modulation of their ability to produce medically and pharmaceutically, important proteins, and the application of animal cells to gene therapy and artificial organs. This volume gives the readers a complete review of present state of the art in Japan. The Proceedings will be useful for cell biologists, biochemists, molecular biologists, immunologists, biochemical engineers and other disciplines related to animal cell culture, working in either academic environments or in biotechnology and pharmaceutical industries.

Animal cell technology aims to understand structures, functions, and behaviors of differentiated animal cells and to ascertain their abilities to be used in industrial and medical purposes. This volume gives readers a complete review of the present state of the art in Japan.