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Zea mays is the most economically important annual crop. A vector
containing GUS reporter gene was introduce into corn via
Agrobacterium mediated transformation.Transformants of different
varieties of maize will be efficiently produced.The transformants
will be histochemically detected for the presence of GUS reporter
gene.Some conditions for best Agrobacterium mediated transformation
was standardized using Agrobacterium tumefaciens strain EHA101
harboring binary vector Pzy102 containing GUS as reporter gene in
kashmiri variety of corn. Numerous factors were compared and
optimized for effecting the transfer of T-DNA and its incorporation
to the genomic system of corn such as immature embryos of 2.5mm
size were analyzed as explants of choice,100 M acetosyringone
concentrations, N6 ad MS media composition, Clorox concentrations
of 30%, co-cultivation durations of 3 days under dark at 22 C and
bacterial concentrations was adjusted to get OD550 0.36, GUS
chemical composition and incubation temperature of 37 C during GUS
analysis were assessed as best. Different optimal experimental
conditions were establish to enhance the -glucuronidase (GUS)
activity after transformation.
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