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Ethanolic extract of Xanthium indicum plant was prepared and tested for its phytochemical and pharmacological investigation. Preliminary phytochemical screening showed that extracts possessed the active principles-alkaloid, carbohydrate, glycoside, flavonoid & Tannin. The extract showed antioxidant property(IC50 ==136.45 ug/mL) compare to standard (ascorbic acid, IC50 =12.16 ug/mL). The extract also shows antioxidant activity by reducing FeCl3. The extract inhibited the acetic acid induced writhing in mice (47.5% P
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