Plant tissue culture (PTC) is basic to all plant biotechnologies and is an exciting area of basic and applied sciences with considerable scope for further research. PTC is also the best approach to demonstrate the totipotency of plant cells, and to exploit it for numerous practical applications. It offers technologies for crop improvement (Haploid and Triploid production, In Vitro Fertilization, Hybrid Embryo Rescue, Variant Selection), clonal propagation (Micropropagation), virus elimination (Shoot Tip Culture), germplasm conservation, production of industrial phytochemicals, and regeneration of plants from genetically manipulated cells by recombinant DNA technology (Genetic Engineering) or cell fusion (Somatic Hybridization and Cybridization). Considerable work is being done to understand the physiology and genetics of in vitro embryogenesis and organogenesis using model systems, especially Arabidopsis and carrot, which is likely to enhance the efficiency of in vitro regeneration protocols. All these aspects are covered extensively in the present book. Since the first book on Plant Tissue Culture by Prof. P.R. White in 1943, several volumes describing different aspects of PTC have been published. Most of these are compilation of invited articles by different experts or proceedings of conferences. More recently, a number of books describing the Methods and Protocols for one or more techniques of PTC have been published which should serve as useful laboratory manuals. The impetus for writing this book was to make available a complete and up-to-date text covering all basic and applied aspects of PTC for the students and early-career researchers of plant sciences and plant / agricultural biotechnology. The book comprises of nineteen chapters profusely illustrated with self-explanatory illustrations. Most of the chapters include well-tested protocols and relevant media compositions that should be helpful in conducting laboratory experiments. For those interested in further details, Suggested Further Reading is given at the end of each chapter, and a Subject and Plant Index is provided at the end of the book.

During the last two decades the modern techniques of histochemistry, electron microscopy, plant physiology, biochemistry, cell and molecular biology, immunology, and genetics have been applied to investigate the intricacies of the processes involved in embryo formation, and considerable new information has been generated. A better understanding of these processes has enhanced our capacity to manipulate fertilization and embryo development. This has changed the face of the embryology of angiosperms from a descriptive science to an experimental and applied science. The revolutionary progress made in this fascinating field of sexual reproduction was the motivation to prepare this volume. It includes 21 chapters written by experts who have made substantial contributions to their respective fields. It covers all aspects of the embryology of angiosperms, ranging from development, isolation, and structure of male and female gametes, their fusion in vivo and in vitro, and structure, physiology, and genetics of zygotic embryogenesis, to endosperm and seed development. Advances in somatic embryogenesis, synthetic seed technology and regeneration of haploid plants from male and female gametophytes are discussed. Other important topics covered in this volume are sexual incompatibility, parthenocarpy, and apomixis. The last chapter deals with the embryological perspective of inheritance of extra-nuclear genes. All the chapters contain up-to-date information and are profusely illustrated. Graduate and postgraduate students, teachers, and scientists of botany and other areas of plant sciences will find this book extremely useful.

This book presents a detailed analysis of up-to-date literature on in vitro morphogenesis at cell, tissue, organ, and whole plant levels. Its driving force is the substantial advances made in the field of morphogenesis in tissue cultures during the last 25 years.

This book presents a detailed analysis of up-to-date literature on in vitro morphogenesis at cell, tissue, organ, and whole plant levels. Its driving force is the substantial advances made in the field of morphogenesis in tissue cultures during the last 25 years.

During the last two decades the modern techniques of histochemistry, electron microscopy, plant physiology, biochemistry, cell and molecular biology, immunology, and genetics have been applied to investigate the intricacies of the processes involved in embryo formation, and considerable new information has been generated. A better understanding of these processes has enhanced our capacity to manipulate fertilization and embryo development. This has changed the face of the embryology of angiosperms from a descriptive science to an experimental and applied science. The revolutionary progress made in this fascinating field of sexual reproduction was the motivation to prepare this volume. It includes 21 chapters written by experts who have made substantial contributions to their respective fields. It covers all aspects of the embryology of angiosperms, ranging from development, isolation, and structure of male and female gametes, their fusion in vivo and in vitro, and structure, physiology, and genetics of zygotic embryogenesis, to endosperm and seed development. Advances in somatic embryogenesis, synthetic seed technology and regeneration of haploid plants from male and female gametophytes are discussed. Other important topics covered in this volume are sexual incompatibility, parthenocarpy, and apomixis. The last chapter deals with the embryological perspective of inheritance of extra-nuclear genes. All the chapters contain up-to-date information and are profusely illustrated. Graduate and postgraduate students, teachers, and scientists of botany and other areas of plant sciences will find this book extremely useful.