Parasitic, bacterial and viral agents continue to challenge the welfare of humans, livestock, wild life and plants worldwide. The public health impact and financial consequences of these diseases are particularly hard on the already overburdened economies of developing countries especially in the tropics. Many of these disease agents utilize insect hosts (vectors) to achieve their transmission to mammals. In the past, these diseases were largely controlled by insecticide-based vector reduction strategies. Now, many of these diseases have reemerged in the tropics, recolonizing their previous range, and expanding into new territories previously not considered to be endemic. Habitat change, irrigation practices, atmospheric and climate change, insecticide and drug resistance as well as increases in global tourism, human traffic and commercial activities, have driven the reemergence and spread of vector borne diseases. While these diseases can be controlled through interventions aimed at both their vertebrate and invertebrate hosts, no effective vaccines exist, and only limited therapeutic prospects are available for their control in mammalian hosts. Molecular technologies such as transgenesis, which is the subject of this book, stand to increase the toolbox and benefit disease management strategies.

Technology for modifying the genotypes and phenotypes of insects and other arthropods has steadily progressed with the development of more precise and powerful methods, most prominently transgenic modification. For many insect pests, there is now almost unlimited ability to modify phenotypes to benefit human health and agriculture. Precise DNA modifications and gene drive have the power to make wild-type populations less harmful in ways that could never have been performed with previous transgenic approaches. This transition from primarily laboratory science to greater application for field use has also necessitated greater development of modeling, ethical considerations and regulatory oversight. The 2nd Edition of Transgenic Insects contains chapters contributed by experts in the field that cover technologies and applications that are now possible. This edition includes increased attention to associated challenges of risk assessment, regulation, and public engagement. Featuring: Up-to-date analysis of molecular techniques, such as gene editing. Consideration of public attitudes and regulatory aspects associated with transgenic insects. Many examples of the wide range of applications of transgenic insects. This book will be very valuable to students and researchers in entomology, molecular biology, genetics, public health and agriculture, and will also appeal to practitioners who are implementing the technology, and to regulators, stakeholders and ethicists.

Arthropod Vector: Controller of Disease Transmission, Volume 1: Vector Microbiome and Innate Immunity of Arthropods is built on topics initially raised at a related Keystone Symposium on Arthropod Vectors. Together with the separate, related Volume 2: Vector Saliva-Host Pathogen Interactions, this work presents a logical sequence of topic development that leads to regulatory considerations for advancing these and related concepts for developing novel control measures. The three themes of symbionts, vector immune defenses and arthropod saliva modulation of the host environment are central to the concept of determinants of vector competence that involves all aspects of vector-borne pathogen development within the arthropod that culminates in the successful transmission to the vertebrate host. These three areas are characterized at the present time by rapid achievement of significant, incremental insights, which advances our understanding for a wide variety of arthropod vector species, and this work is the first to extensively integrate these themes.

Arthropod Vector: Controller of Disease Transmission, Volume 2: Vector Saliva-Host Pathogen Interactions is built on topics initially raised at a related Keystone Symposium on Arthropod Vectors. Together with the separate, related Volume 1: Controller of Disease Transmission, this work presents a logical sequence of topic development that leads to regulatory considerations for advancing these and related concepts for developing novel control measures. The three themes of symbionts, vector immune defenses and arthropod saliva modulation of the host environment are central to the concept of determinants of vector competence that involves all aspects of vector-borne pathogen development within the arthropod that culminates in the successful transmission to the vertebrate host. These three areas are characterized at the present time by rapid achievement of significant, incremental insights, which advances our understanding for a wide variety of arthropod vector species, and this work is the first to extensively integrate these themes.

Parasitic, bacterial and viral agents continue to challenge the welfare of humans, livestock, wild life and plants worldwide. The public health impact and financial consequences of these diseases are particularly hard on the already overburdened economies of developing countries especially in the tropics. Many of these disease agents utilize insect hosts (vectors) to achieve their transmission to mammals. In the past, these diseases were largely controlled by insecticide-based vector reduction strategies. Now, many of these diseases have reemerged in the tropics, recolonizing their previous range, and expanding into new territories previously not considered to be endemic. Habitat change, irrigation practices, atmospheric and climate change, insecticide and drug resistance as well as increases in global tourism, human traffic and commercial activities, have driven the reemergence and spread of vector borne diseases. While these diseases can be controlled through interventions aimed at both their vertebrate and invertebrate hosts, no effective vaccines exist, and only limited therapeutic prospects are available for their control in mammalian hosts. Molecular technologies such as transgenesis, which is the subject of this book, stand to increase the toolbox and benefit disease management strategies.