Plant-parasitic and free-living nematodes are increasingly important in relation to food security, quarantine measures, ecology (including pollution studies), and research on host-parasite interactions. Being mostly microscopic, nematodes are challenging organisms for research. Techniques for Work with Plant and Soil Nematodes introduces the basic techniques for laboratory and field work with plant-parasitic and free-living soil-dwelling nematodes. Written by an international team of experts, this book is extensively illustrated, and addresses both fundamental traditional techniques and new methodologies. The book covers areas that have become more widespread over recent years, such as techniques used in diagnostic laboratories, including computerized methods to count and identify nematodes. Information on physiological assays, electron microscopy techniques and basic information on current molecular methodologies and their various applications is also included. This book is an essential resource for students of nematology and parasitology, academic researchers, diagnostic laboratories, and quarantine and advisory service personnel. It provides a much-needed methodology standard for anyone involved in work on plant and soil nematodes.

Plant-parasitic nematodes are recognized as one of the greatest threats to crop production throughout the world. Estimated annual crop losses of $8 billion in the United States and $78 billion worldwide are attributed to plant parasitic nematodes. Plant parasitic nematodes not only cause damage individually but form disease-complexes with other microorganisms thereby increasing crop loss. Nematode diseases of crops are difficult to control because of their insidious nature and lack of specific diagnostic symptoms which closely resemble those caused by other plant pathogens and abiotic diseases. Future developments of sustainable management systems for preventing major economical agricultural losses due to nematodes is focused on strategies that limit production costs, enhance crop yields, and protect the environment. This book presents a first compendium and overview for nematode problems and their management across North America. Each chapter provides essential information on the occurrence and distribution of plant parasitic nematodes, their major crop hosts, impact on crop production and sustainable management strategies for each region of the continent including, Canada, Mexico and all states of the USA. For each region, a thematic overview of changes in crop production affected by plant parasitic nematodes and their management strategies over time will provide invaluable information on the important role of plant parasitic nematodes in sustainable agriculture.