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This book presents the fundamentals and applications of Matrix
Assisted Laser Desorption/Ionization Time-of-Flight Mass
Spectrometry (MALDI-ToF-MS) technique. It highlights the basic
principles, the history of invention as well as the mechanism of
ionization and mass determination using this technique. It
describes the fundamental principles and methods for MALDI spectra
interpretation and determination of exact chemical structures from
experimental data. This book guides the reader through the
interpretation of MALDI data where complex macromolecular spectra
are simplified in order to present the major principles behind data
interpretation. In addition, each chapter describes how
MALDI-ToF-MS analysis provides necessary understanding of the
copolymer systems that have been designed for specialized
biomedical applications.
This book offers comprehensive information on all aspects of ELISA,
starting with the fundamentals of the immune system. It also
reviews the history of analytical assays prior to the advent of
ELISA (enzyme-linked immunosorbent assay) and addresses the
materials of choice for the fabrication of the platforms, possible
biomolecular interactions, different protocols, and evaluation
parameters. The book guides readers through the respective steps of
the analytical assay, while also familiarizing them with the
possible sources of error in the assay. It offers detailed insights
into the immobilization techniques used for protein attachment, as
well as methods for evaluating the assay and calculating the key
parameters, such as sensitivity, specificity, accuracy and limit of
detection. In addition, the book explores the advantages and
shortcomings of the conventional ELISA, as well as various
approaches to improving its performance. In this regard, merging
and integrating other technologies with widely known ELISAs have
opened new avenues for the advancement of this immunoassay.
Accordingly, the book provides cutting-edge information on
integrated platforms such as ELISpot, plasmonic ELISAs,
sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in
micro-devices.
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