This second edition provides new and updated protocols to investigate clock-controlled parameters including period estimations and entrainment measurements and modelling. Chapters detail promoter activity using luciferase reporters, fluorescent-tagging of clock proteins and cellular imaging, protein level measurements, regulated RNA splicing detection, post-translational modifications, protein-protein interactions, and protein DNA/chromatin interactions. Additional chapters focus on seasonal processes like hypocotyl growth and flowering time control in annuals and trees, and techniques on soybean. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and practical, Plant Circadian Networks: Methods and Protocols, Second Edition is designed not only for the chronobiology community dealing with circadian biology but also for the plant community in general. "Period estimation and rhythm detection in timeseries data using BioDare2, the free, online, community resource" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

What makes a coach great? How do great coaches turn a collection of individuals into a coherent "us"? Seth Davis, one of the keenest minds in sports journalism, has been thinking about that question for twenty-five years. It's one of the things that drove him to write the definitive biography of college basketball's greatest coach, John Wooden, Wooden: A Coach's Life. But John Wooden coached a long time ago. The world has changed, and coaching has too, tremendously. Seth Davis decided to embark on a proper investigation to get to the root of the matter. In Getting to Us, Davis probes and prods the best of the best from the landscape of active coaches of football and basketball, college and pro-from Urban Meyer, Dabo Swinney, and Jim Harbaugh to Mike Krzyzewski, Tom Izzo, Jim Boeheim, Brad Stevens, Geno Auriemma, and Doc Rivers-to get at the fundamental ingredients of greatness in the coaching sphere. There's no single right way, of course-part of the great value of this book is Davis's distillation of what he has learned about different types of greatness in coaching, and what sort of leadership thrives in one kind of environment but not in others. Some coaches have thrived at the college level but not in the pros. Why? What's the difference? Some coaches are stern taskmasters, others are warm and cuddly; some are brilliant strategists but less emotionally involved with their players, and with others it's vice versa. In Getting to Us, we come to feel a deep connection with the most successful and iconic coaches in all of sports-big winners and big characters, whose stories offer much of enduring interest and value.

Fiduciary law is important transnationally, particularly in the context of global capitalism. Fiduciary law's characteristic regard for others offers a response to the pursuit of unconstrained self-interest in business and government relations, potentially implicating the exercise of both private and public power. Stakeholders have invoked it not only to address traditional private law matters, but also to enjoin transnational corporations to respect human rights, to combat public corruption, and to constrain national governments to respect the rights of Indigenous Peoples. This book focuses on the processes through which conceptualizations of fiduciary relationships and fiduciary norms may (or may not) settle transnationally - or become unsettled - as actors invoke fiduciary norms to address problems in different domains, including across borders. It identifies complications and challenges of any transnational convergence of fiduciary norms that fiduciary theorists often elide. This book is also available as Open Access on Cambridge Core.

A powerful memoir from the University of Kentucky basketball legend, NBA veteran, and social media influencer about his recovery from addiction. He is considered by many the greatest basketball player ever produced by the hoops-crazy state of Kentucky. In two years at the University of Kentucky, he scored over 1,000 points, led the Wildcats to a Sweet Sixteen appearance and was nicknamed "King Rex." The first player ever drafted by the Charlotte Hornets, he spent twelve seasons in the NBA, dazzling in dunk contests and sinking one of the most memorable buzzer-beaters in league history. But by the end of his career, Rex Chapman was harboring a destructive secret. Years before America's opioid crisis would become national news, Chapman developed a dependency on Vicodin and Oxycontin, ultimately ingesting fifty painkillers a day. In addition, he developed a severe gambling addiction, once nearly losing $400,000 at a Las Vegas blackjack table. All this would cost him his family as well as most of the $40 million fortune he'd made in basketball, leaving him to live in his car and shoplift to support his addictions. Only when he was arrested--and his mugshot made national news--did he finally commit to getting clean. In It's Hard for Me to Live With Me, Chapman--who has amassed millions of social media followers for his relatable and uplifting posts--tells the story of his addiction and recovery in unflinching detail. With equal frankness, he describes his history with depression; the racism he witnessed growing up and how that shaped his outspokenness on matters of social justice; and his complex and volatile relationship with his father, also a former professional basketball player. Cowritten with New York Times bestselling author Seth Davis, Chapman's memoir is an equally devastating and inspiring story about the human struggle for self-acceptance.

This second edition provides new and updated protocols to investigate clock-controlled parameters including period estimations and entrainment measurements and modelling. Chapters detail promoter activity using luciferase reporters, fluorescent-tagging of clock proteins and cellular imaging, protein level measurements, regulated RNA splicing detection, post-translational modifications, protein-protein interactions, and protein DNA/chromatin interactions. Additional chapters focus on seasonal processes like hypocotyl growth and flowering time control in annuals and trees, and techniques on soybean. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and practical, Plant Circadian Networks: Methods and Protocols, Second Edition is designed not only for the chronobiology community dealing with circadian biology but also for the plant community in general. "Period estimation and rhythm detection in timeseries data using BioDare2, the free, online, community resource" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

A provocative and revelatory new biography of the legendary UCLA coach John Wooden, by one of America's top college basketball writers

No college basketball coach has ever dominated the sport like John Wooden. His UCLA teams reached unprecedented heights in the 1960s and '70s capped by a run of ten NCAA championships in twelve seasons and an eighty-eight-game winning streak, records that stand to this day. Wooden also became a renowned motivational speaker and writer, revered for his "Pyramid of Success."

Seth Davis of "Sports Illustrated" and CBS Sports has written the definitive biography of Wooden, an unflinching portrait that draws on archival research and more than two hundred interviews with players, opponents, coaches, and even Wooden himself. Davis shows how hard Wooden strove for success, from his All-American playing days at Purdue through his early years as a high school and college coach to the glory days at UCLA, only to discover that reaching new heights brought new burdens and frustrations. Davis also reveals how at the pinnacle of his career Wooden found himself on questionable ground with alumni, referees, assistants, and even some of his players. His was a life not only of lessons taught, but also of lessons learned.

Woven into the story as well are the players who powered Wooden's championship teams - Kareem Abdul-Jabbar, Bill Walton, Walt Hazzard, and others - many of whom speak frankly about their coach. The portrait that emerges from Davis's remarkable biography is of a man in full, whose life story still resonates today.