With the surprising development across the cosmetic and personal care companies the re-utilization of cosmetic tools is of a common practice. Isolation and detection of human skin pathogens from 100 samples of beauty salon tools i.e., blusher brush, face sponge and wax has been done. All the samples were examined microbiologically for the contamination of Staphylococcus aureus, Pseudomonas aeruginosa, yeast and fungus. It was observed that the percentage of Staphylococcus aureus was higher (100% in sponge, 100% in brush, 88% in wax) in the tools than Pseudomonas aeruginosa (69.6% in sponge, 81.8% in brush and 73.5% in wax), where counts obtained for fungus was 51.5% in sponge, 30.3% in brush and 20.5% in wax. It was observed that the major cause of contamination of saloon tools is repetitive usage on all costumers without considering the hygienic conditions.

Vinca rosea L. Syn. Catharanthus roseus L. G. Don. is an important medicinal plant which belongs to the family Apocynaceae. The present study deals with Micropropagation, Callogenesis, Cell suspension culture & Antimicrobial activity of Vinca rosea L.. Explants were cultured on MS basal medium and supplemented with different concentrations of BAP (mg/l) and NAA (mg/l) for micropropagation whereas 2, 4-D (mg/l) and Kin (mg/l) for callogenesis. Multiple shoots were obtained on all the concentrations of BAP and NAA, but BAP (1 mg/l) showed best response (90% & 80%).Similarly best callus response was observed on MS medium supplemented with 2, 4- D + Kin (1+1 mg/l) in all the explants (95% from leaf, 80% from node & 60% from fruit). Cell suspension culture have also been established in the present study according to which the calluses gave suspensions consisting of both the embryogenic and non-embryogenic cluster of the cells in 2, 4- D + Kin (1+1 mg/l) and gave best response (70%).The present study also describes the comparison of in vivo and in vitro antimicrobial activity of Vinca rosea. Three different pathogenic bacterial strains .) and three different fungal strains were used.

Medicinal plants are important source for the verification of pharmacological effects and can be natural composite sources that act as new anti-infectious agent. Different plant parts are used for medicinal purposes i.e., bulb, gel, leaves, roots, barks, peels etc. The use of plants to treat illness is found throughout human culture. According to the report of the world health organization, 80% of the world population relies mainly on traditional therapies which involved the use of plant extracts or their active substances (World Health Organization, 1993).

We performed a retrospective study between December 2010 and May 2011 on all retinitis pigmentosa (RP) cases seen from Okara during ophthalmological consultation at Lyton Rahmatullah Benevolent trust (LRBT) hospital from August 2008 to January 2011. The aim of this study was to determine the preponderance of low vision and blindness in retinitis pigmentosa in patients of Okara. The techniques used for the diagnosis of retinitis pigmentosa were an examination of ocular fundus using a biomicroscope (fundoscopy), far visual acuity and visual field. Indeed, a drop in visual acuity and blindness were the main reasons for consultation in this study. 100 cases were reported of which two were with family history from Okara, 70 male and 30 females were affected from RP. The average age of RP patients was 27.45 with a range of 4-78 years. Blindness and low vision was observed in 54% of the patients and 40% respectively. The average age of RP patients with low vision ranges from 6-50 years and the average age of RP patients with blindness range from 9-65 years. Patients should be screened to enable them to benefit from management focusing on both appropriate treatment and genetic counseling.

Visual impairment and blindness have very important impact on the socioeconomic development of individuals and societies.Present retrospective, analytical study was performed on all retinitis pigmentosa cases seen from Lahore during ophthalmologic consultation at the Layton Rahmatullah Benevolent trust (LRBT) hospital Lahore.The aim of this research was to determine the predominance of visual disability associated with retinitis pigmentosa in Lahore.

Research work on floriculture is being carried out at several research institutions. The crops which have received larger attention include rose, gladiolus, chrysanthemum, orchid, jasmine, tuberose, aster, marigold etc. The thrust till recently had been on crop improvement, standardization of agro-techniques including improved propagation methods, plant protection and post harvest management. In view of the fact that most of the cut flower production is being done under open field conditions, the research efforts generally relate to open cultivation. In recent years, however, technologies for protected cultivation and tissue culture for mass propagation have also received attention. A large number of varieties suitable for cut flower use, as well as garden display have been developed. Production technology, particularly the agronomic requirements and control methods for important diseases.