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Efficacies of locally isolated bacterium, Bacillus sphaericus total
proteins have been investigated against the 3rd instar larvae of
wild mosquito Culex quinquefasciatus under the laboratory
conditions.The LC50 values were recorded as 19.069 ug/ul at 72 h
posttreatment. Association of both the proteins of 42 kDa and 51
kDa were found to require for mosquitocidal efficacy. The thermal
stability properties of the Bin proteins were performed at various
temperature ranges from 40 C, 50 C, 60 C, 70 C, 80 C & 90 C.
The efficacy of the heat incubated Bin proteins were tested and
showed that the binary proteins were active up to 70 C but lost
their activity when treated at 80 C and 90 C. Among the two Bin
proteins, 42 kDa proteins were found to be more stable than the 51
kDa protein. This data suggest that both the proteins were required
for mosquitocidal efficacy for the production of bioinsectiside
which would be used as a biological control of mosquito."
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