Within Pyrosequencing Protocols, the protocols for utilizing Pyrosequencing technology are described in detail, including trouble-shooting tips and background information. Chapter 1 provides an introduction to the fascinating origins of the methodology. Chapter 2 provides a brief overview of some of the applications Pyrosequencing is used for. Chapters 3 and 4 describe primer selection and the basic technique. Chapters 5 through 7 provide methods for improving the throughput and decreasing the cost of Pyrosequencing. Detailed applications for the technique can be ound in Chapters 8-13, whilst the important aspect of data storage is discussed in Chapter 14.

This book presents detailed protocols for the multidisciplinary application of Pyrosequencing(r) technology, all written by world-renowned experts. This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing(r), and supplementing each protocol with troubleshooting tips specific to that method. This volume both highlights the versatility of and provides detailed protocols for the application of Pyrosequencing(r).