This book constitutes selected revised papers of the 16th International Conference on Formalizing Natural Languages: Applications to Natural Language Processing and Digital Humanities, NooJ 2022, held in Rosario, Argentina, in June 2022. Due to COVID-19 pandemic the conference was held virtually. NooJ is a linguistic development environment that provides tools for linguists to construct linguistic resources that formalize a large gamut of linguistic phenomena: typography, orthography, lexicons for simple words, multiword units and discontinuous expressions, inflectional, derivational and agglutinative morphology, local, phrase-structure and dependency grammars, as well as transformational and semantic grammars. The 17 full papers presented were carefully reviewed and selected from 50 submissions. The papers are organized in the following topics: Morphological and Lexical Resources; Syntactic and Semantic Resources; Corpus Linguistics and Discourse Analysis; Natural Language Processing Applications.