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This second edition provides new and updated chapters useful for
the study of Regulatory B cells. Organized in four sections,
chapters detail basic methods for the isolation and
immunophenotypical analysis of these cells, experimental approaches
for the ex vivo generation/expansion of IL-10 producing B cells,
molecular biology techniques for the analysis of IL-10 expression
and production, and animal models mimicking pathologic settings.
Written in the highly successful Methods in Molecular Biology
series format, chapters include an introduction to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, as well as
tips on troubleshooting and avoiding known pitfalls. Authoritative
and cutting-edge, Regulatory B Cells: Methods and Protocols, Second
Edition aims to be useful to the scientific community and serve to
clarify some unsolved aspects of Regulatory B Cells research.
This second edition provides new and updated chapters useful for
the study of Regulatory B cells. Organized in four sections,
chapters detail basic methods for the isolation and
immunophenotypical analysis of these cells, experimental approaches
for the ex vivo generation/expansion of IL-10 producing B cells,
molecular biology techniques for the analysis of IL-10 expression
and production, and animal models mimicking pathologic settings.
Written in the highly successful Methods in Molecular Biology
series format, chapters include an introduction to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, as well as
tips on troubleshooting and avoiding known pitfalls. Authoritative
and cutting-edge, Regulatory B Cells: Methods and Protocols, Second
Edition aims to be useful to the scientific community and serve to
clarify some unsolved aspects of Regulatory B Cells research.
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