Since the publication of the popular first edition, genomic methods have become more accessible, allowing antibiotic researchers to probe not only the sequence of antibiotic resistance determinants but the mechanism whereby they are expressed and regulated. That, in concert with array technology and an understanding of the importance of biofilms, has greatly expanded antibiotic resistance knowledge. In order to reflect the growing field, Antibiotic Resistance Protocols, Second Edition fully updates and builds upon its first edition with contributions from leading researchers. Beginning with chapters on epidemiology and population genetics, the book continues with sections covering genomics and gene expressions, fitness mutation and physiology, and the detection of resistance. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Antibiotic Resistance Protocols, Second Edition brings together examples of a diverse range of modern techniques applied in antibiotic research in order to best aid scientists in planning their own future research.

This third edition provides a wide range of different technologies, ranging from conventional growth basic techniques, application of molecular biology, development of resistance mutations, and diagnosis and monitoring treatment response. New and updated chapters cover techniques from the microscopic scale to whole animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Antibiotic Resistance Protocols, Third Edition aims to ensure successful results in the further study of this vital field.

A comprehensive and cutting-edge review of how practicing physicians can best treat multiple drug resistance in bacterial, viral, protozoal, and helminthic infections. The authors focus on the major hospital and community-acquired pathogens, including S. aureus, S. pneumoniae, Enterococcus, Acinetobacter, and M. tuberculosis, and on the management of such common problems as multiple drug-resistant urinary tract infections and gonorrhea. Among the resistant tropical organisms covered are Salmonella typhi, malaria, and Burkholderia pseudomalei. Resistance to such important antiviral classes as antiretrovirals and anticytomegalovirals is also discussed, as are those measures necessary to prevent the spread of infections patients.

Since the publication of the popular first edition, genomic methods have become more accessible, allowing antibiotic researchers to probe not only the sequence of antibiotic resistance determinants but the mechanism whereby they are expressed and regulated. That, in concert with array technology and an understanding of the importance of biofilms, has greatly expanded antibiotic resistance knowledge. In order to reflect the growing field, Antibiotic Resistance Protocols, Second Edition fully updates and builds upon its first edition with contributions from leading researchers. Beginning with chapters on epidemiology and population genetics, the book continues with sections covering genomics and gene expressions, fitness mutation and physiology, and the detection of resistance. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Antibiotic Resistance Protocols, Second Edition brings together examples of a diverse range of modern techniques applied in antibiotic research in order to best aid scientists in planning their own future research.

A comprehensive and cutting-edge review of how practicing physicians can best treat multiple drug resistance in bacterial, viral, protozoal, and helminthic infections. The authors focus on the major hospital and community-acquired pathogens, including S. aureus, S. pneumoniae, Enterococcus, Acinetobacter, and M. tuberculosis, and on the management of such common problems as multiple drug-resistant urinary tract infections and gonorrhea. Among the resistant tropical organisms covered are Salmonella typhi, malaria, and Burkholderia pseudomalei. Resistance to such important antiviral classes as antiretrovirals and anticytomegalovirals is also discussed, as are those measures necessary to prevent the spread of infections patients.