This book comprises selected papers of the 25th International Conference on Difference Equations and Applications, ICDEA 2019, held at UCL, London, UK, in June 2019. The volume details the latest research on difference equations and discrete dynamical systems, and their application to areas such as biology, economics, and the social sciences. Some chapters have a tutorial style and cover the history and more recent developments for a particular topic, such as chaos, bifurcation theory, monotone dynamics, and global stability. Other chapters cover the latest personal research contributions of the author(s) in their particular area of expertise and range from the more technical articles on abstract systems to those that discuss the application of difference equations to real-world problems. The book is of interest to both Ph.D. students and researchers alike who wish to keep abreast of the latest developments in difference equations and discrete dynamical systems.