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This volume is comprised of a collection of experimental protocols
for common techniques and strategies used to study the biogenesis
of b-barrel outer membrane proteins in Gram-negative bacteria. The
BAM Complex: Methods and Protocols guides readers through methods
on the function of the BAM complex, the roles played by each of the
individual components, the expression and purification of the
components, crystallization and structure determination of the
components, and how the individual Bam components may assemble into
a functional complex. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, The BAM Complex: Methods and
Protocols will serve as an invaluable reference for those
interested in studying the BAM complex.
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