This work provides general methods for determining protein structure, from the simple electrophoretic, chromatographic, and spectrophotometric techniques to the relatively more complex and sophisticated methods of mass spectrometry and ultracentrifugation. This second edition has been updated to embrace the developments in the field, and should be of value to all involved in protein research. It is intended for graduates and post-doctoral researchers, academic staff, biotechnology professionals in the field of protein science protein structure is also of relevance to biochemists.