Scientific Study from the year 2012 in the subject Biology - Micro- and Molecular Biology, Indian Institute of Science, course: Molecular Biology, language: English, comment: This is a tool to separate compounds according to its hydrophobicity or hydrophilicity. A gradient is given from hydrophilic to hydrophobic range of solvent moving through a solid matrix. Here are some valuable tips collected through experience and handed over so that the reader can find it useful when the chromatograms and the instruments prove so cross with you. The instrumentation and working for both the systems HPLC and FPLC are almost same. HPLC is High Performance/Pressure Liquid Chromatography and FPLC is Fast Protein Liquid Chromtography., abstract: This is a tool to separate compounds according to its hydrophobicity or hydrophilicity. A gradient is given from hydrophilic to hydrophobic range of solvent moving through a solid matrix. Here are some valuable tips collected through experience and handed over so that the reader can find it useful when the chromatograms and the instruments prove so cross with you. The instrumentation and working for both the systems HPLC and FPLC are almost same. HPLC is High Performance/Pressure Liquid Chromatography and FPLC is Fast Protein Liquid Chromtography.

Scholarly Research Paper from the year 2011 in the subject Biology - Micro- and Molecular Biology, printed single-sided, grade: A, Indian Institute of Science, course: Microbial Ecology - bioremediation of contaminated subsurface systems, language: English, comment: Using natural biofilms to degrade contaminants in subsurface systems by in depth studies in simulated Winogradsky systems, abstract: Everything is everywhere. The environment selects. Practical applications possible out of winogradsky column like using it as a universal enrichment medium for all microbes to grow as they are and also to isolate and evolve purpose based microbes for degradation studies. Nature is just a hidden Winogradsky column. Thus whatever mechanisms that underlies degradation in field is the same when simulated inside a winogradsky column. So we simulate all the constraints that face the degradation of hard substrates and the mechanisms inside this column to achieve results that can be immediately applied in field, without need of elaborate experimentation with artificial culture medium.

Scholarly Research Paper from the year 2011 in the subject Biology - Micro- and Molecular Biology, grade: A, Indian Institute of Science, course: Research Project - Microbe Fuel Cells, language: English, comment: Rechargable LED light from microbes (Microbe Fuel Cells, Supercapacitors, Resistors and LEDs), abstract: Biogeochemical cycling through microbial succession in Winogradsky columns generates electricity. Here we use all electricals tools possible to harness this electricity and power Light Emitting Diodes for a year or more. The setup, biochemical mechanisms are analysed.

Scholarly Research Paper from the year 2011 in the subject Chemistry - Bio-chemistry, Indian Institute of Science, course: Research Project, language: English, comment: A lot of work is being done in Biofilms, but how about growing natural biofilms in the most natural way possible?, abstract: We can harness the potential of a Winogradsky column in making a large tank with natural biofilm forming simulated conditions, including wind and waves. Over a period of time this environment provides natural biogeochemical cycles and microbial succession to take place resulting in biofilms as of those found in a water body like a lake. We can then study the biofilm forming tendencies of soil microflora in a very convenient and natural way.