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This book summarizes the latest findings on the functions of
microRNAs in the regulation of plant development and responses to
the surrounding environment. MicroRNAs are an important class of
molecules that can be found in diverse groups of organisms,
including plants and animals, and the investigation of their roles
is a highly dynamic and "hot" research topic. The respective
chapters address four main aspects, namely: microRNA investigation
and annotation, the regulatory roles of microRNAs in various
developmental processes, in response to abiotic factors, and in the
context of biotic stress response regulation. Systematically
reviewing the most important findings in this field, the book
offers an essential guide for undergraduate and graduate students,
teachers, and plant science researchers. Due to the potential
applications of microRNAs in crop breeding and plant protection, it
also represents a valuable resource for scientists in academia and
the private sector alike.
This volume is a collection of miRNA detection and target
identification protocols, detailing new developments in the
traditional detection approaches such as northern blot,
quantitative real-time PCR, array, next generation sequencing, and
in situ hybridization. The chapters in MicroRNA Detection and
Target Identification: Methods and Protocols guide readers through
novel approaches such as nanotechnology, microfluidics, based
detection methods, analysis of serum and urinary, miRNAs as
biomarkers, target identification and experimental approaches.
Written in the highly successful Methods in Molecular Biology
series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Authoritative and
practical, MicroRNA Detection and Target Identification: Methods
and Protocols aims to ensure successful results in the further
study of this vital field.
This volume is a collection of miRNA detection and target
identification protocols, detailing new developments in the
traditional detection approaches such as northern blot,
quantitative real-time PCR, array, next generation sequencing, and
in situ hybridization. The chapters in MicroRNA Detection and
Target Identification: Methods and Protocols guide readers through
novel approaches such as nanotechnology, microfluidics, based
detection methods, analysis of serum and urinary, miRNAs as
biomarkers, target identification and experimental approaches.
Written in the highly successful Methods in Molecular Biology
series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Authoritative and
practical, MicroRNA Detection and Target Identification: Methods
and Protocols aims to ensure successful results in the further
study of this vital field.
Since the discovery of microRNAs, developmental biologists have
striven to understand the role of miRNAs in development and
disease. MicroRNAs in Development: Methods and Protocols collects
contributions from expert researchers in order to provide practical
guidelines to this complex study. Divided into three convenient
sections, this detailed volume covers various techniques to detect
and profile miRNA expression, followed by protocols to manipulate
the activity of miRNAs in various organisms, and it concludes with
a section that outlines different methods to identify and validate
miRNA targets in animals and plants. Written in the highly
successful Methods in Molecular Biology (TM) series format,
chapters contain introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and notes on troubleshooting and
avoiding known pitfalls. Authoritative and accessible, MicroRNAs in
Development: Methods and Protocols serves as a practical guide for
scientists of all backgrounds and conveys the appropriate sense of
fascination associated with this vital field of research.
Since the discovery of microRNAs, developmental biologists have
striven to understand the role of miRNAs in development and
disease. MicroRNAs in Development: Methods and Protocols collects
contributions from expert researchers in order to provide practical
guidelines to this complex study. Divided into three convenient
sections, this detailed volume covers various techniques to detect
and profile miRNA expression, followed by protocols to manipulate
the activity of miRNAs in various organisms, and it concludes with
a section that outlines different methods to identify and validate
miRNA targets in animals and plants. Written in the highly
successful Methods in Molecular Biology (TM) series format,
chapters contain introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and notes on troubleshooting and
avoiding known pitfalls. Authoritative and accessible, MicroRNAs in
Development: Methods and Protocols serves as a practical guide for
scientists of all backgrounds and conveys the appropriate sense of
fascination associated with this vital field of research.
This updated volume reflects new and evolved techniques to study
detection, profiling, and manipulation of microRNAs (miRNAs) in
plants and animals. After overviews of how best to detect,
identify, and validate microRNAs, the book continues by exploring
state-of-the-art protocols for microRNA detection, approaches to
profile the expression level of microRNAs, spatial expression
analysis, describe in silico analysis of microRNAs and their
targets, as well as protocols for functional analysis of microRNAs
and their targets by CRISPR/Cas. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions
to their respective topics, lists of the necessary materials and
reagents, step-by-step and readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, MicroRNA Detection and Target
Identification: Methods and Protocols, Second Edition aims to
ensure successful results in the further study of this vital field.
Plant gene silencing is a crucially important phenomenon in gene
expression and epigenetics. This book describes the way small RNA
is produced and acts to silence genes, its likely origins in
defence against viruses, and also its potential to improve plants.
Plant gene silencing can be used to improve industrial traits, make
plants more nutritious or more valuable to consumers, to remove
allergens, and to improve resistance to weeds and pathogens.
This book summarizes the latest findings on the functions of
microRNAs in the regulation of plant development and responses to
the surrounding environment. MicroRNAs are an important class of
molecules that can be found in diverse groups of organisms,
including plants and animals, and the investigation of their roles
is a highly dynamic and "hot" research topic. The respective
chapters address four main aspects, namely: microRNA investigation
and annotation, the regulatory roles of microRNAs in various
developmental processes, in response to abiotic factors, and in the
context of biotic stress response regulation. Systematically
reviewing the most important findings in this field, the book
offers an essential guide for undergraduate and graduate students,
teachers, and plant science researchers. Due to the potential
applications of microRNAs in crop breeding and plant protection, it
also represents a valuable resource for scientists in academia and
the private sector alike.
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R1,150
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