Over the past decades, knowledge about the cellular and molecular basis underlying the visual process has remarkably increased. In Retinal Degeneration: Methods and Protocols, expert researchers in the field provide a guide of relevant and state-of-the-art methods for studying retinal homeostasis and disease. These include methods and techniques for addressing cell culture systems and animal models of disease, their generation, their phenotypic and molecular characterization as well as their use in therapeutic approaches to the retina. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Retinal Degeneration: Methods and Protocols aids scientists in continuing to study the cutting-edge techniques of retinal cell biology in health and disease.

In the 1920s, actor George Valentin is a bona fide matinee idol with many adoring fans. While working on his latest film, George finds himself falling in love with an ingenue named Peppy Miller and, what's more, it seems Peppy feels the same way. But George is reluctant to cheat on his wife with the beautiful young actress.

The growing popularity of sound in movies further separates the potential lovers, as George's career begins to fade while Peppy's star rises.

Academy Award Winner
- Best Picture Of 2011
- Best Director
- Best Actor
- Best Costume Design
- Best Musical Score

This volume provides key updates on several methods from the first edition as well as including new novel techniques to address the most recent technological developments and their applications in retinal research Chapters guide readers through gene identification approaches, detailed protocols to generate functional retinal pigment epithelium cells, mouse retina and other animal models, fundus imaging and angiography, and cell-based treatment approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Retinal Degeneration: Methods and Protocols, Second Edition aims to ensure successful scientific work in the further study of this vital field.

Over the past decades, knowledge about the cellular and molecular basis underlying the visual process has remarkably increased. In Retinal Degeneration: Methods and Protocols, expert researchers in the field provide a guide of relevant and state-of-the-art methods for studying retinal homeostasis and disease. These include methods and techniques for addressing cell culture systems and animal models of disease, their generation, their phenotypic and molecular characterization as well as their use in therapeutic approaches to the retina. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Retinal Degeneration: Methods and Protocols aids scientists in continuing to study the cutting-edge techniques of retinal cell biology in health and disease.

Michel Hazanavicius writes and directs this modern-day silent film comedy recounting the demise of the silent film industry in the late 1920s. Jean Dujardin takes on the role of George Valentin, one of the biggest stars of the silent movie era. George seems to have the perfect life: he loves his work, enjoys adoration from fans and falls in love with a beautiful young starlet, Peppy Miller (Bérénice Bejo), after working with her on a movie. When studio boss Zimmer (John Goodman) warns him that the future of film making lies in 'talkies', George is dismissive of the threat. However, as films with audible dialogue begin to take off - with Peppy the undoubted star of the new medium - George struggles to keep pace with a changing world. The film was nominated for ten Oscars at the 2012 Academy Awards and won five, including Best Picture, Best Director and Best Actor (Dujardin).

Elijah Wood stars in this remake of director William Lustig's 1980 horror classic of the same name. To say that Frank (Wood) is a troubled young man would be an understatement - as the ever-growing number of young women he has murdered since the death of his mother (America Olivo), with whom he enjoyed an unhealthily close relationship, demonstrates only too well. Frank runs a mannequin store and likes to apply the scalps of his victims to the models in his spare time. However, when beautiful young artist Anna (Nora Arnezeder) wanders into the store and talks about making use of its resources for an exhibition she is planning, she presents Frank with a troubling quandary. Anna appears to offer the possibility of a human relationship that will fill the void created by his mother's death, but she also excites his desire to kill and possess. Will Frank be able to overcome his homicidal urges?