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Die uberarbeitete und aktualisierte 7. Auflage dieses Buches gibt
einen UEberblick uber bewahrte und neue Methoden der
Proteinbiochemie und Proteomics. Es zeigt Auswege aus
experimentellen und strategischen Sackgassen. Zudem weckt es ein
Gespur fur das richtige Experiment zur richtigen Zeit. Behandelt
werden klassische Verfahren wie Saulenchromatographie, HPLC,
Elektrophoresen, Blots, ELISA, Ligandenbindungstests, die
Herstellung von Antikoerpern, das Solubilisieren von
Membranproteinen, die Analyse von Glykoproteinen usw. Einen grossen
Raum nehmen die modernen Verfahren ein: Massenspektrometrie,
Proteomics und thermische Analyse. In die 7. Auflage wurden neue
Techniken zur Bestimmung der Wechselwirkung von Proteinen mit
Proteinen oder von Proteinen mit kleinen Molekulen aufgenommen:
DARTS, DRACALA, SPROX und andere. Des weiteren erfahren Sie, wie
man mit dem Massenspektrometer eine Bindung misst. Auch Methoden
zur Herstellung von Bindungsproteinen gegen bestimmte Zielmolekule
werden vorgestellt: Ribosomen Display und DNA- und
Peptid-Aptamer-Techniken. Der Fluoreszenznachweis von Proteinen mit
Hilfe von Trihalogenverbindungen durfte nicht fehlen und wer die
Stabilitat und Faltung von Proteinen messen will, kann hier
nachlesen, ob er dazu ein CD-Spektrometer benutzen sollte. Auf die
Fortschritte in der HPLC und der Massenspektrometrie von
Membranproteinen wird ebenso eingegangen wie auf ihre
Rekonstitution in Nanoscheibchen (Nanodiscs). Die Mikrodissektion
mit UV-Laser, die isoelektrische Fokussierung in Kapillaren und
iTRAQ-Tags werden erklart. Dazu kommt eine Anzahl neuer Tricks zur
Proteinbestimmung, Gelfarbung, Blottechnik, Immunfarbung, Elution
aus Gelstuckchen etc.
This book is the first example in presenting LC-MS strategies for
the analysis of peptides and proteins with detailed information and
hints about the needs and problems described from experts
on-the-job. The best advantage is -for sure- the practical insight
of experienced analysts into their novel protein analysis
techniques. Readers starting in 'Proteomics' should be able to
repeat each experiment with own equipment and own protein samples,
like clean-up, direct protein analysis, after (online) digest, with
modifications and others. Furthermore, the reader will learn more
about strategies in protein analysis, like quantitative analysis,
industrial standards, functional analysis and more.
Experiencing Mathematics: Activities to Engage the High School
Student is the result of the collaborative effort of nine
Adolescent Young Adult (AYA) National Board Certified Teachers
(NBCTs) in mathematics. This student edition is a compilation of
successful activities that prompt high school students to explore,
conjecture, and reflect on their mathematical adventures-thus
"experience" mathematics. The relevant nature of the activities
will motivate students to pursue their investigations with vigor
and take the intellectual risk necessary to construct knowledge and
improve problem-solving competence. This book provides student
activities grouped in algebra, geometry, and algebra 2 sections
that parallel the traditional sequencing in major texts. Through
activity design, students are lead through an inductive inquiry in
which they conjecture, formulate, and test ideas and concepts. This
inductive student-centered approach to learning will not only
excite students but also increase student effort.
Experiencing Mathematics: Activities to Engage the High School
Student is the result of the collaborative effort of nine
Adolescent Young Adult (AYA) National Board Certified Teachers in
mathematics. This teacher edition is a compilation of successful
activities that prompt high school students to explore, conjecture,
and reflect on their mathematical adventures thus "experience"
mathematics. The relevant nature of the activities will motivate
students to pursue their investigations with vigor and take the
intellectual risk necessary to construct knowledge and improve
problem-solving competence. This edition will educate teachers
regarding tenets of cognitive science, motivation theory, and the
NCTM/NBCT standards. It will dispel the myth that the crowded
curriculum does not have room for such activities and, in fact,
will convince teachers that using such activities increases teacher
and student motivation and achievement. The resources provide
detailed instructions for each activity, along with student prompts
and reflective questions for the teacher.
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