This second edition volume expands on the previous edition with new information on the structural analysis of glycosaminoglycans (GAGs); chemical and enzymatic synthesis of GAGs; biophysical, biochemical, and computational analysis of GAG-protein interactions; molecular and genetic approaches to manipulating GAG cell biology; and molecular and genetic methods involving animal models and organoids. This volume also offers insight and guidance to non-glycoscience researchers who are performing advanced experiments on GAGs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. \ Cutting-edge and authoritative, Glycosaminoglycans: Methods and Protocols, Second Edition is a valuable experimental manual for practicing researchers including graduate and post-doctoral fellows, and provides them with the latest methodologies and practical tips to overcoming barriers in understanding the chemistry and biology of GAGs.

A pioneering and comprehensive work, The Singapore Blue Chips puts the spotlight on 22 of Singapore's largest corporates. This is the first book that provides a quick snapshot of Singapore's large cap (large market capitalisation) corporates as investment propositions, and is a timely tribute to the nation's 50 years of independence and development.Written for finance professionals and students as well as readers with a general interest in business, investing and finance, each chapter of this book is dedicated to one company and delves into its attractiveness as an investment proposition, the associated investments risk and the company's prospects as of end-2016.

A pioneering and comprehensive work, The Singapore Blue Chips puts the spotlight on 22 of Singapore's largest corporates. This is the first book that provides a quick snapshot of Singapore's large cap (large market capitalisation) corporates as investment propositions, and is a timely tribute to the nation's 50 years of independence and development.Written for finance professionals and students as well as readers with a general interest in business, investing and finance, each chapter of this book is dedicated to one company and delves into its attractiveness as an investment proposition, the associated investments risk and the company's prospects as of end-2016.

This second edition volume expands on the previous edition with new information on the structural analysis of glycosaminoglycans (GAGs); chemical and enzymatic synthesis of GAGs; biophysical, biochemical, and computational analysis of GAG-protein interactions; molecular and genetic approaches to manipulating GAG cell biology; and molecular and genetic methods involving animal models and organoids. This volume also offers insight and guidance to non-glycoscience researchers who are performing advanced experiments on GAGs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. \ Cutting-edge and authoritative, Glycosaminoglycans: Methods and Protocols, Second Edition is a valuable experimental manual for practicing researchers including graduate and post-doctoral fellows, and provides them with the latest methodologies and practical tips to overcoming barriers in understanding the chemistry and biology of GAGs.