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Monoclonal Antibodies: Methods and Protocols, Second Edition
expands upon the previous edition with current, detailed modern
approaches to isolate and characterize monoclonal antibodies
against carefully selected epitopes. This edition includes new
chapters covering the key steps to generate high quality
monoclonals via different methods, from antigen generation to
epitope mapping and quality control of the purified IgG. Chapters
are divided into four parts corresponding to four distinct
objectives. Part I covers monoclonal antibody generation, Part II
deals with monoclonal antibody expression and purification, Part
III presents methods for monoclonal antibody characterization and
modification, and Part IV describes selected applications of
monoclonal antibodies. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Monoclonal Antibodies: Methods and
Protocols, Second Edition provides crucial initial steps of
monoclonal antibody generation and characterization with
state-of-the art protocols.
Monoclonal Antibodies: Methods and Protocols, Second Edition
expands upon the previous edition with current, detailed modern
approaches to isolate and characterize monoclonal antibodies
against carefully selected epitopes. This edition includes new
chapters covering the key steps to generate high quality
monoclonals via different methods, from antigen generation to
epitope mapping and quality control of the purified IgG. Chapters
are divided into four parts corresponding to four distinct
objectives. Part I covers monoclonal antibody generation, Part II
deals with monoclonal antibody expression and purification, Part
III presents methods for monoclonal antibody characterization and
modification, and Part IV describes selected applications of
monoclonal antibodies. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Monoclonal Antibodies: Methods and
Protocols, Second Edition provides crucial initial steps of
monoclonal antibody generation and characterization with
state-of-the art protocols.
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