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After the identification of a potential protein drug, the next
critical step is the production of sufficient authentic material
for testing, characterization, and clinical trials, which, when
successful, leads to the need for robust methodologies for
large-scale production, purification, characterization, viral
inactivation, and continued testing of the final protein product.
Building on the valuable first edition, Therapeutic Proteins:
Methods and Protocols, Second Edition aims to cover each of these
key aspects of protein drug production through the contributions of
authors from highly esteemed industrial and academic institutions
around the world. Emphasizing the newest developments in the field,
this second edition also includes additional emphasis on discovery,
including new display and screening methods as well as the design
and engineering of new types of therapeutic proteins. There is also
discussion of computational and bioinformatics methods, and
chapters on safety aspects of therapeutic protein development.
Written in the highly successful Methods in Molecular Biology (TM)
format, protocol chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Fully updated and
practical, Therapeutic Proteins: Methods and Protocols, Second
Edition provides an essential resource to all scientists working in
the field of therapeutic proteins.
After the identification of a potential protein drug, the next
critical step is the production of sufficient authentic material
for testing, characterization, and clinical trials, which, when
successful, leads to the need for robust methodologies for
large-scale production, purification, characterization, viral
inactivation, and continued testing of the final protein product.
Building on the valuable first edition, Therapeutic Proteins:
Methods and Protocols, Second Edition aims to cover each of these
key aspects of protein drug production through the contributions of
authors from highly esteemed industrial and academic institutions
around the world. Emphasizing the newest developments in the field,
this second edition also includes additional emphasis on discovery,
including new display and screening methods as well as the design
and engineering of new types of therapeutic proteins. There is also
discussion of computational and bioinformatics methods, and
chapters on safety aspects of therapeutic protein development.
Written in the highly successful Methods in Molecular Biology (TM)
format, protocol chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Fully updated and
practical, Therapeutic Proteins: Methods and Protocols, Second
Edition provides an essential resource to all scientists working in
the field of therapeutic proteins.
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