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This book provides a single-source reference on the current state
of the ribosome profiling method by describing experimental
protocols for the quantitative analysis of translation in a variety
of model organisms. In addition, the volume presents a detailed
overview of the existing software tools and includes detailed
description of methods for statistical analysis, data processing,
and visualization of ribosome profiling data. Written for the
highly successful Methods in Molecular Biology series, chapters
include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Authoritative and practical, Ribosome
Profiling: Methods and Protocols aims to provide the type of
standardized protocols that have previously been unavailable in an
effort to bypass the major barriers to wide use of ribosome
profiling-based approaches.
This book provides a single-source reference on the current state
of the ribosome profiling method by describing experimental
protocols for the quantitative analysis of translation in a variety
of model organisms. In addition, the volume presents a detailed
overview of the existing software tools and includes detailed
description of methods for statistical analysis, data processing,
and visualization of ribosome profiling data. Written for the
highly successful Methods in Molecular Biology series, chapters
include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Authoritative and practical, Ribosome
Profiling: Methods and Protocols aims to provide the type of
standardized protocols that have previously been unavailable in an
effort to bypass the major barriers to wide use of ribosome
profiling-based approaches.
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