This detailed volume collects protocols from scientists who are actively engaged in developing or using protoplast technology. The book begins with chapters that focus on basic protoplast techniques and their utilities, such as protocols on protoplast isolation, transfection, and regeneration, as well as examples of how to use protoplasts for genome editing and gene function analysis in a number of major crop or model plant species. It continues by exploring protoplast automation, large scale functional genomics, and synthetic biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protoplast Technology: Methods and Protocols aims to inspire the new generation of researchers to further improve their protocols and apply this technology to accelerate the field of plant genomic study.

As the most populous country in the world, China's demographic challenges have always been too many people for ecological system, resources, and the environment. However, by the early 1990s, fertility rate in China had dropped below the replacement level, and China's low fertility has now attracted the world's attention. This book is among the first studies to raise and examine questions on low fertility in China, believing that China has entered a new era featured by low birth rate and ageing population. Utilizing advanced research methods and models on low fertility to analyze China's census data, this book explores the issues from various perspectives. Methodologies employed in past population studies, policy making concerning fertility rate, underreporting of births and fertility rate estimates, fertility level of the migrant population, current population pattern, long-term population trends, population dynamics, and many other thought-provoking problems are covered. Finally, the book revisits China's population issues in the context of globalization. The 21st century has seen the new challenge of persistent population decrease and ageing worldwide, which, along with economic globalization, demands a new understanding of the changes in population pattern and their consequences. Researchers and students in China's demographic and social studies will be attracted by the insightful analysis and rich materials provided in the book. Population policy makers will also benefit from it.

As the most populous country in the world, China's demographic challenges have always been too many people for ecological system, resources, and the environment. However, by the early 1990s, fertility rate in China had dropped below the replacement level, and China's low fertility has now attracted the world's attention. This book is among the first studies to raise and examine questions on low fertility in China, believing that China has entered a new era featured by low birth rate and ageing population. Utilizing advanced research methods and models on low fertility to analyze China's census data, this book explores the issues from various perspectives. Methodologies employed in past population studies, policy making concerning fertility rate, underreporting of births and fertility rate estimates, fertility level of the migrant population, current population pattern, long-term population trends, population dynamics, and many other thought-provoking problems are covered. Finally, the book revisits China's population issues in the context of globalization. The 21st century has seen the new challenge of persistent population decrease and ageing worldwide, which, along with economic globalization, demands a new understanding of the changes in population pattern and their consequences. Researchers and students in China's demographic and social studies will be attracted by the insightful analysis and rich materials provided in the book. Population policy makers will also benefit from it.

This volume brings together a number of in-depth studies on Asian population history. The chapters discuss a diverse range of subjects -- comparative perspectives, fertility, disease and mortality, and marriage and family -- over a wide geographic area -- Japan, China, Taiwan, Indonesia, India, and Sri Lanka. This volume offers plenty of material for comparative study and will particularly appeal to academics and students in the fields of demography, history, and Asian studies.

Based on research into jets in supersonic crossflow carried out by the authors' team over the past 15 years, this book summarizes and presents many cutting-edge findings and analyses on this subject. It tackles the complicated mixing process of gas jets and atomization process of liquid jets in supersonic crossflow, and studies their physical mechanisms. Advanced experimental and numerical techniques are applied to further readers' understanding of atomization, mixing, and combustion of fuel jets in supersonic crossflow, which can promote superior fuel injection design in scramjet engines. The book offers a valuable reference guide for all researchers and engineers working on the design of scramjet engines, and will also benefit graduate students majoring in aeronautical and aerospace engineering.

Establishing multi-scale optimal water allocation modes which takes the enhancement of utilization efficiency as core is a research hotspot for the international water resources management of river basins. This book aims to introduce how to compile the first set of county-level IO tables involving resources and environment accounts with integrated datasets which contains the spatio-temporal data of water and land resources, ecology and social economy in the river basin and to construct an integrated Computable General Equilibrium (CGE) model with resources and environment accounts embedded, which can be used to quantitatively depict the key process parameters of the water-ecology-social economy coupling system. Thus, this book can provide decision support for integrated river basin management, and scientific support for the sustainable development of social economy, eco-environment and water resources.

This detailed volume collects protocols from scientists who are actively engaged in developing or using protoplast technology. The book begins with chapters that focus on basic protoplast techniques and their utilities, such as protocols on protoplast isolation, transfection, and regeneration, as well as examples of how to use protoplasts for genome editing and gene function analysis in a number of major crop or model plant species. It continues by exploring protoplast automation, large scale functional genomics, and synthetic biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Protoplast Technology: Methods and Protocols aims to inspire the new generation of researchers to further improve their protocols and apply this technology to accelerate the field of plant genomic study.

The book lies at the interface of mathematics, social media analysis, and data science. Its authors aim to introduce a new dynamic modeling approach to the use of partial differential equations for describing information diffusion over online social networks. The eigenvalues and eigenvectors of the Laplacian matrix for the underlying social network are used to find communities (clusters) of online users. Once these clusters are embedded in a Euclidean space, the mathematical models, which are reaction-diffusion equations, are developed based on intuitive social distances between clusters within the Euclidean space. The models are validated with data from major social media such as Twitter. In addition, mathematical analysis of these models is applied, revealing insights into information flow on social media. Two applications with geocoded Twitter data are included in the book: one describing the social movement in Twitter during the Egyptian revolution in 2011 and another predicting influenza prevalence. The new approach advocates a paradigm shift for modeling information diffusion in online social networks and lays the theoretical groundwork for many spatio-temporal modeling problems in the big-data era.

This book constitutes the refereed proceedings of the First International Workshop on Machine Learning and Medical Engineering for Cardiovasvular Healthcare, MLMECH 2019, and the International Joint Workshops on Computing and Visualization for Intravascular Imaging and Computer Assisted Stenting, CVII-STENT 2019, held in conjunction with MICCAI 2019, in Shenzhen, China, in October 2019. For MLMECH 2019, 16 papers were accepted for publication from a total of 21 submissions. They focus on machine learning techniques and analyzing of ECG data in the diagnosis of heart diseases. CVII-STENT 2019 accepted all 8 submissiones for publication. They contain technological and scientific research concerning endovascular procedures.

This is a new release of the original 1927 edition.

Kessinger Publishing is the place to find hundreds of thousands of rare and hard-to-find books with something of interest for everyone!

Kessinger Publishing is the place to find hundreds of thousands of rare and hard-to-find books with something of interest for everyone!

Kessinger Publishing is the place to find hundreds of thousands of rare and hard-to-find books with something of interest for everyone!

This book presents new evidence about historical and contemporary Chinese population behavior that overturns much of the received wisdom about the differences between China and the West first voiced by Malthus. Malthus described a China in which early and universal marriage ensured high fertility and therefore high mortality. He contrasted this with Western Europe, where marriage was late and far from universal, resulting in lower fertility and higher demographic responsiveness to economic circumstances. The result in China was thought to be mass misery as part of the population teetered on the brink of a Malthusian precipice, whereas in the West conditions were less severe.

In reality, James Lee and Wang Feng argue, there has been effective regulation of population growth in China within marriage through a variety of practices that depressed marital fertility to levels far below European standards and through the widespread practices of infanticide and abortion. Moreover, in China population control has long been primarily a consequence of collective intervention. This collective culture underlies the four distinctive features of the Chinese demographic pattern -- high rates of female infanticide, low rates of male marriage, low rates of marital fertility, and high rates of adoption -- that Lee and Wang trace from 1700 to today. These and other distinctive features of the Chinese demographic and social system, they argue, led to a different demographic transition in China from the one that took place in the West.