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This detailed volume presents a variety of animal models that are
commonly used to study sepsis and some key procedures to measure
specific disease outcomes. The chapters describe well-established
surgical and nonsurgical rodent models of sepsis, presented by
experts in the field. In addition, the book includes protocols for
burn injury and sepsis, modeling the mouse intensive care unit
(MICU), and the development of humanized mice, which may be useful
tools to increase the translational potential of rodent sepsis
research. There is a chapter discussing the use of biomarkers for
sepsis diagnosis and prognosis in humans and mice, as well as
chapters describing specific methods for biomarker measurement, and
the use of analgesics and humane endpoints in rodent sepsis
research. Finally, agent-based computational modeling is presented
as a valuable complementary approach to study sepsis. Written for
the highly successful Methods in Molecular Biology series, chapters
include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Authoritative and practical, Sepsis:
Methods and Protocols serves as an ideal guide for researchers
working toward improving the identification and treatment of septic
patients.
This detailed volume presents a variety of animal models that are
commonly used to study sepsis and some key procedures to measure
specific disease outcomes. The chapters describe well-established
surgical and nonsurgical rodent models of sepsis, presented by
experts in the field. In addition, the book includes protocols for
burn injury and sepsis, modeling the mouse intensive care unit
(MICU), and the development of humanized mice, which may be useful
tools to increase the translational potential of rodent sepsis
research. There is a chapter discussing the use of biomarkers for
sepsis diagnosis and prognosis in humans and mice, as well as
chapters describing specific methods for biomarker measurement, and
the use of analgesics and humane endpoints in rodent sepsis
research. Finally, agent-based computational modeling is presented
as a valuable complementary approach to study sepsis. Written for
the highly successful Methods in Molecular Biology series, chapters
include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Authoritative and practical, Sepsis:
Methods and Protocols serves as an ideal guide for researchers
working toward improving the identification and treatment of septic
patients.
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